重组腺病毒介导HSV-TK基因对胶质瘤细胞的生长抑制  

The Growth Inhibition of Glioma Cell by Adenovirus-mediated HSV-TK Gene

作　　者：史国利[1] 房欣[1] 王金环[1] 冯宇新 罗勇[1] 陈德风[1] 

机构地区：[1]南开大学分子生物学研究所分子遗传室,300071

出　　处：《中国临床神经科学》1999年第1期10-12,共3页Chinese Journal of Clinical Neurosciences

摘　　要：本文构建了带有HSV-TK基因的腺病毒包装质粒pACCMVTK,并获得重组腺病毒AdCMVTK,用其感染胶质母细胞瘤细胞系TJ905,研究了HSV-TK/GCV系统对该细胞系的生长影响。结果发现该系统对胶质母细胞瘤细胞系TJ905生长有明显的抑制作用,以100MOI(重复感染率)的病毒剂量感染细胞,GCV半数抑制浓度IC_(50)仅为5μmol,同时发现HSV-TK/GCV系统对TJ905细胞系有明显的旁观效应。表明该系统有治疗胶质瘤的应用前景。The plasmid pACCMVTK that expresses simple virus thymidine kinase (HSV-TK) gene was constructed, and recombinant adnovirus AdCMVTK was gained. Glioblastoma cells TJ905 were infected by AdCMVTK. The effect of HSV-TK/GCV system to growth of glioma cell was observed. The results showed HSV-TK/GCV can inhibit the growth of TJ905 cells efficiently, the IC50 was 5 μM when 100 MOI(multipicity of infection) recombinant adenovirus AdCMVTK infected TJ905 cells. Otherwise, a strong 'bystander effect' of HSV-TK/GCV system was noted in tumor cell lines. The study indicated that AdCMVTK/GCV system may be used in treatment of glioma in the future.

关 键 词：重组腺病毒 HSV-TK基因 胶质母细胞瘤 抑瘤作用 

分 类 号：R739.410.5[医药卫生—肿瘤] R730.51[医药卫生—临床医学]