人原代胶质瘤细胞自杀基因治疗体外研究  

Study HSV tk gene mediated killing of human glioma

作　　者：陶慧敏 李承晏[1,2,1] 余绍祖[1,2,1] 喻文莉[1,2,1] 

机构地区：[1]湖北医科大学附属第一医院神经科 [2]湖北医科大学附属同仁医院神经科

出　　处：《卒中与神经疾病》1999年第1期9-10,共2页Stroke and Nervous Diseases

基　　金：湖北省科委资助

摘　　要：目的：应用原代培养的胶质瘤细胞作不研究对象，观察ＡＣＶ对转染了ＨＳＶ┐ｔｋ基因的肿瘤细胞的杀伤作用。方法：表达单纯疱疹病毒胸苷激酶（ＨＳＶ┐ｔｋ）重组逆转录病毒载体ＳＴＫ，经包装细胞ＰＡ３１７包装成重组逆转录病毒，ＮＩＨ３Ｔ３细胞测定病毒滴度，用此病毒感染原代培养的人胶质瘤细胞，以Ｇ４１８选择后获得ＴＫ＋肿瘤细胞。ＭＴＴ法及活细胞动态观察ＡＣＶ对转基因细胞的毒性作用。结果：ＡＣＶ对ｔｋ＋胶质瘤细胞的杀伤作用明显高于ｔｋ－细胞（ｔｋ＋与ｔｋ－细胞存活率差值ｔ检验Ｐ＜０．００２），随着ＡＣＶ浓度的增高，ｔｋ＋胶质瘤细胞的死亡率明显高于ｔｋ－细胞（两直线斜率比较ｔ检验Ｐ＜０．００１）。结论：逆转录病毒载体转染了ＨＳＶ┐ｔｋ基因的原代胶质瘤细胞可选择性的被ＡＣＶ杀死。Objective:The human primary cultrue glioma.Methods:The HSV tk gene was transfered into primary culture glioma cell by retroviral vector.The cellular morphology and the killing effect of ACV were observed in both tk+ glioma cell and tk- glioma cell.Results:The results showed that tk+ glioma cell was significantly killed by ACV compared with nontransfected cell ( P <0.002) and the killing effect increased as the ACV concentration increased ( P <0.001).Conclusion:The human primary culture glioma transfected with HSV tk gene by retroviral vector can be killed by ACV selectively.

关 键 词：单纯疱疹病毒 胸苷激酶 基因疗法 胶质瘤 

分 类 号：R730.264[医药卫生—肿瘤] R730.51[医药卫生—临床医学]