从木瓜成叶中提取齐墩果酸及其羟丙基-β-环糊精包合工艺研究  

Extraction of Oleanolic Acid from Leaves of Chaenomeles spciosa and Processing Techniques of Its HP-β-cyclodextrin Inclusion Compound

作　　者：陈秋实[1] 吴杰[1] 李威[1] 成蓓[2] 

机构地区：[1]武汉生物工程学院制药工程系,湖北武汉430415 [2]武汉大学药学院,湖北武汉430070

出　　处：《中药材》2010年第5期804-807,共4页Journal of Chinese Medicinal Materials

基　　金：武汉市重点科研课题(200718)

摘　　要：目的:优选木瓜成叶中齐墩果酸的最佳提取条件及其羟丙基-β-环糊精包合工艺。方法:采用正交实验法,以超声波处理时间、提取时间、提取温度、料液比为影响因素,以齐墩果酸得率为指标优选提取工艺。然后采用正交实验法,以羟丙基-β-环糊精和齐墩果酸投料比、包合时间、包合温度为影响因素,以包合物收得率和齐墩果酸包合率的综合评分为指标,优选包合工艺。结果:最佳提取条件为超声波处理时间40min,提取时间70min,提取温度60℃,料液比1∶10。最佳包合条件为羟丙基-β-环糊精和齐墩果酸投料比1∶2,包合时间3.0h,包合温度60℃。结论:按照本实验方法可得到较高的提取率及得到稳定的包合物。Objective：To select the best extraction of oleanolic acid from leaves of Chaenomeles spciosa and processing techniques of its HP-β-cyclodextrin（HP-β-CD）inclusion compound.Methods：The study was carried out with orthogonal experiment.Ultrasonic treatment time,extraction time,temperature of extraction and ratio of material to liquor were used as indices.The optimum inclusion technique was chosen by determining the oleanolic acid-bearing rate.The processing techniques of the HP-β-CD inclusion compound was optimized by taking the ratio of HP-β-CD to oleanolic acid,the time and temperature of the inclusion process as factors and with the yield of the inclusion compound and the inclusion rate of the volatile oil as the indices.Results：The optimal process for oleanolic acid extraction were as following：ultrasonic treatment time as 40 min,extraction time as 70 min,temperature of extraction as 60 ℃,ratio of material to liquor as 1∶10.The optimal processing conditions for HP-β-CD inclusion compound of oleanolic acid were as following：the ratio of HP-β-CD to oleanolic acid was 1∶2,the inclusion time of the compound was 3.0 hours at 60 ℃.Conclusion：High extraction yield of oleanolic acid and stable inclusion compound can be obtained using the above method.

关 键 词：木瓜成叶 齐墩果酸 正交实验 提取 羟丙基-Β-环糊精 

分 类 号：R943[医药卫生—药剂学]