胃癌超声内镜分期与微小卫星DNA不稳定性关系的研究  

MICROSATELLITE INSTABILITY AND ITS RELATION TO GASTRIC CANCER STAGING BY ENDOSCOPIC ULTRASONOGRAPHY

作　　者：郭文[1] 张亚历[1] 邱红明[1] 吴保平[1] 张立力[1] 周殿元[1] 

机构地区：[1]第一军医大学南方医院消化科

出　　处：《中国癌症杂志》1999年第1期4-6,共3页China Oncology

基　　金：军队95重点课题基金

摘　　要：目的探讨微小卫星ＤＮＡ不稳定性（ＭＩＮ）与胃癌术前分期的关系。方法应用超声内镜对７３例胃癌患者行术前分期，同时采用银染ＰＣＲＳＳＣＰ方法检测内镜活检组织第２、５、１７号染色体４个位点的ＭＩＮ。结果ＭＩＮ总的检出率为３７０％，其中出现４、３、２个及单个位点异常分别为１１１％、２２２％、４８１％和１８５％。Ｔ１至Ｔ４期癌ＭＩＮ检出率分别为４０４％、３３３％、３９３％和３５７％（Ｐ＞００５），与胃癌的浸润深度无关，其中有淋巴结转移组为５１４％，明显高于无转移组的２２２％（Ｐ＜００２５）。结论ＭＩＮ是胃癌病变中常见的分子遗传学事件，与胃癌转移有一定的相关性。PURPOSE To observe the relationship between endoscopic ultrasonography(EUS) staging and microsatellite instability (MIN) of gastric carcinoma.METHODS 73 cases of gastric carcinoma were taken for the preoperative staging with EUS. Silver staining PCR SSCP method was used to detect MIN at 4 loci on chromosome 2、5、17 in the 73 paraffin embedded biospy specimens.RESULTS The abnormal shifting of the single stranded DNA was identified in 27 cases,the overall detective rate of MIN was 37.0%.Of these 27 tumors,MIN at all 4,3,2 loci or one locus was 11.1%,22.2%,48.1% and 18.5%,respetively.The detective rate of MIN had no relation with the depth of invasion,which was 40.0%,33.3%,39.3% and 35.7% at T 1 to T 4 stage ( P >0 05),respectively,but it was significantly higher in cases with lymph node metastasis(51.4%) than those without metastasis (22.2%) ( P <0 025).CONCLUSION MIN is a common molecular hereditary event in gastric carcinoma,and it may be one of the important mechanisms of gastric carcinogenesis and metastasis.

关 键 词：胃癌 超声内镜 肿瘤分期 微小卫星DNA 不稳定性 

分 类 号：R735.204[医药卫生—肿瘤]