人canstatin基因对人食管癌裸鼠移植瘤中bcl-xl和cyclin D1表达的影响及意义  

Study on Effect and Significance of Canstatin Gene on Expressions of bcl-xl and Cyclin D1 on Human Esophageal Carcinoma Xenografts in Nude Mice

作　　者：卢高峰[1] 李贵[1] 马军[1] 唐芙爱[1] 

机构地区：[1]郑州大学第二附属医院消化内科,郑州市450014

出　　处：《医药论坛杂志》2010年第12期36-39,共4页Journal of Medical Forum

摘　　要：目的探讨人canstatin基因对人食管癌裸鼠移植瘤的的影响作用,寻求其抑制肿瘤生长的机制。方法 KYSE150细胞建立裸鼠食管癌动物模型,Ad-canstatin实施干预,RT-PCR检测肿瘤组织bcl-xl、cy-clinD1的mRNA的表达水平。免疫组织化学方法检测微血管密度(MVD)。结果治疗组肿瘤体积明显小于两个对照组,差异有统计学意义(P<0.05),而两对照组间差异无统计学意义。对电泳结果进行灰度分析和统计学分析显示canstatin治疗组cyclinD1、bcl-xl的mRNA表达明显低于GFP组和PBS组,差异均有统计学意义(P<0.05)。canstatin治疗组MVD明显低于对照组。结论人canstatin基因对人食管癌移植瘤的生长具有抑制作用,其作用机制可能与抑制cyclin D1、bcl-xl表达而抑制肿瘤血管生成有关。Objective This study was to investigate the antitumor effects of canstatin gene on human esophageal carcinoma xenografts in nude mice.Methods Esophageal cancer celles were implanted into BALB/c nude mice to induce tumor xenografts,tumor xenografts were randomized into three groups： PBS,adenovirus green fluorescent protein（Ad-GFP）,and Ad-GFP-canstatin groups.After treatment,tumor size was measured.The expression of cyclin D1、bcl-xl mRNA Ievels were detected by RT-PCR and microvessel density（MVD） in tissue of each group were detected by immunohistochemistry.Results Compared with that in Ad-GFP and PBS groups,tumor size in Ad-GFP-canstatin group was significantly suppressed after gene transfection.Compared with Ad-GFP and PBS groups,expressions of cyclinD1 and bcl-xl in Ad-GFP-canstatin group was lower（P0.05）,and microvessel density（MVD） were lower too（P0.05）.Conclusion Canstatin can inhibit the growth of human esophageal carcinoma by suppressing angiogenesis via down-regulating cyclin D1 and bcl-xl expression.

关 键 词：CANSTATIN 食管癌 BCL-XL CyclinD1 

分 类 号：R-332[医药卫生]