绿脓杆菌制剂对人肝癌MHCC97L细胞增殖和侵袭能力的影响  

Effects of Pseudomonas aeruginosa vaccine on proliferation and invasiveness of hepatocellular carcinoma cell line MHCC97L

作　　者：周建炜[1] 李涛[2] 任正刚[1] 刘彬彬[1] 黄修燕[1] 居旻杰[1] 汤钊猷[1] 

机构地区：[1]复旦大学肝癌研究所、中山医院,教育部癌变与侵袭原理重点实验室,上海200032 [2]山东大学齐鲁医院普外科,济南250012

出　　处：《中华肝胆外科杂志》2010年第6期455-459,共5页Chinese Journal of Hepatobiliary Surgery

摘　　要：目的 探讨绿脓杆菌制剂（pseudomonas aeruginosa vaccine,PA）对有转移潜能的人肝癌MHCC97L细胞增殖和侵袭能力的影响.方法 采用不同浓度的PA分别作用于MHCC97L细胞,观察细胞增殖、生长曲线、克隆形成率、流式细胞术分析（FCM）、侵袭、运动、迁移、VEGF和MMP2蛋白表达（ELISA法）.结果 PA明显抑制MHCC97L细胞增殖和克隆形成,呈良好的剂量-效应关系.PA 48 h和72 h的IC50分别为3.1×108/ml和1. 9×108/ml.PA浓度为0.5×108/ml、1×108/ml和2×108/ml时,其细胞倍增时间依次增加,克隆形成率依次降低（P均＜0.01）;FCM显示,G1期细胞比例随PA浓度增加而增加,S＋G2期细胞比例随PA浓度增加而降低（P均＜0.01）.PA浓度为1×108/ml时,穿过人工基底膜（侵袭实验）和上室底膜（运动实验）的细胞数（分别为4.8±1.3和8.8±2.2）明显低于对照组（8.6±2.1和15.6±1.2）（P均＜0.01）;细胞经72 h培养后,对照组划痕逐渐愈合,1×108/ml的PA组细胞划痕依然明显.ELISA法检测发现,1×108/ml的PA组其VEGF蛋白和MMP2蛋白含量和对照组相比均无明显差异（P均＞0.05）.结论 在一定条件下,绿脓杆菌制剂可抑制人肝癌MHCC97L细胞增殖和克隆形成,其作用部分是通过使细胞周期阻滞在G1期实现的;绿脓杆菌制剂可以明显抑制MHCC97L细胞的侵袭、运动和迁移能力,其作用和VEGF、MMP2蛋白分泌关系不明显.Objective To investigate the effects of Pseudomonas aeruginosa vaccine （PA） on proliferation and invasiveness of the hepatocellular carcinoma cell line MHCC97L with metastatic potential. Methods Proliferation, growth curve, plate efficiency, flow cytometry, transwell invasion assay, cell motility assay, scarification test, vascular endothelial growth factor （VEGF） and matrix metalloproteinase-2 （MMP2） protein activity were evaluated after cells were treated with PA at various concentrations. Results PA can inhibit the proliferation and plate efficiency of MHCC97L cell markedly in a dose-dependent manner. The IC50 of cells treated with PA for 48 h and 72 h was 3.1 ×108/ml and 1.9 × 108/ml, respectively. The doubling time increased and plate efficiency decreased gradually when cells treated with 0.5 × 108/ml, 1 × 108/ml and 2 × 108/ml PA （P＜0.01）. PA could induce cell cycle arrest at the G1 phase in a dose-dependent manner by flow cytometric analysis. The average amount of invading cell per field in cell invasion assay and motility assay were 4. 8 ± 1.3 and 8. 8±2.2 when cells treated with 1× 108/ml PA, which was significantly lower than that of control group （8. 6±2. 1 and 15. 6±1.2 ） （P＜0.01） Scarification test showed that the metastatic ability of cells treated with 1 × 108/ml PA significantly lower than that in the control group. Comparison between cells treated with 1 × 108/ml PA and control group, no remarkable difference was found regarding expression of VEGF and MMP2 in supernatant of cell culture. Conclusion PA can inhibit proliferation and plate efficiency of HCC cell line MHCC97L, which is in part mediated by the cell cycle arrest at the G1 phase. PA could inhibit invasiveness of HCC cell line MHCC97L, which is unrelated to the VEGF and MMP2 protein activity.

关 键 词：癌 肝细胞 绿脓杆菌制剂 细胞增殖 侵袭性 血管内皮生长因子 金属蛋白酶 

分 类 号：R735.7[医药卫生—肿瘤]