1,25二羟维生素D3联合顺铂对子宫内膜癌HEC-1-A细胞增殖及Skp2/p27表达的影响  

The effect of 1,25(OH)_2D_3 and DDP on proliferation and expression of Skp2/p27 of endometrial carcinoma cell line HEC-1-A

作　　者：林兰[1] 郑秀[2] 余江[1] 江敏[3] 郭彩凤[1] 李素霞[1] 

机构地区：[1]福建中医学院附属第二人民医院妇产科,福州350013 [2]福建中医学院附属第二人民医院中心实验室,福州350013 [3]福建医科大学附属第一医院妇产科

出　　处：《现代妇产科进展》2010年第6期423-426,共4页Progress in Obstetrics and Gynecology

摘　　要：目的:探讨1,25二羟维生素D3[1,25(OH)2D3]与顺铂(DDP)联合抑制人子宫内膜癌HEC-1-A细胞的增殖,测定二者作用下S期激酶相关蛋白(Skp2)、抑癌基因p27蛋白的表达。方法:用CCK-8法测定1,25(OH)2D3、DDP及二者联合处理后细胞增殖情况,用流式细胞仪分析细胞周期,用W estern blot检测Skp2及p27蛋白表达的变化。结果:与对照组相比,1,25(OH)2D3、DDP作用下细胞吸光度(OD)值减少,细胞增殖受到抑制(P<0.01),二者联用时此作用明显增强;1,25(OH)2D3、DDP均能增加G0/G1期细胞比例,降低S、G2/M期细胞比例,从而降低细胞增殖指数(P<0.01);二者作用下Skp2表达减少,p27蛋白表达增多。结论:1,25(OH)2D3有增强DDP抗肿瘤的作用,二者均可能通过下调Skp2、上调p27蛋白表达,抑制癌细胞增殖。Objective：To study the effect of 1,25（OH）2D3 and cisplatin（DDP） on proliferation of human endometrial carcinoma cell line HEC-1-A, and to explore its mechanism by detecting the expressions of S-phase kinase associated protein 2 （Skp2） and p27 protein. Methods：HEC-1-A cells were cultured in vitro and treated with 1,25 （OH）2D3 ,DDP single and combination in vitro. Cell proliferation was evaluated by CCK-8 method,distribution of cell cycle was determined by flow cytometry,and the expression level of Skp2/p27 protein was detected by Western blot. Results ： Compared with the control group, 1,25 （OH） 203 and DDP inhibited the proliferation of HEC-1-A cell line, and the effect was more significant in the combined group. 1,25 （OH）2Dz and DDP increased the rate of G0/G1, decreased the rates of S and G2/M ,and so decreased cells PI（P〈0. 01 ）. Both of them decreased the expression of Skp2 and increased the expression of p27 protein. Conclusion： 1,25 （OH）203 can enhance the inhibitory effect of DDP on cell proliferation. 1,25 （OH）2D3 and DDP may inhibit cancer cell proliferation through down-regulation of Skp2 and up-regulation of p27 protein.

关 键 词：1 25二羟维生素D3 子宫内膜肿瘤 细胞系 顺铂 SKP2 蛋白质P27 

分 类 号：R737.33[医药卫生—肿瘤]