孕期酒精暴露对小鼠视皮质神经元树突棘和突触的影响  

Effects of alcohol exposure during pregnancy on dendritic spine and synapse of visual cortex in filial mice

作　　者：崔占军[1] 赵凯冰[2] 文曙光[1] 张俊士[1] 于东明[1] 邓锦波[1] 

机构地区：[1]河南大学医学院神经生物学研究所,河南开封475004 [2]开封大学医学部,河南开封475001

出　　处：《药学学报》2010年第7期833-839,共7页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金资助项目(30670688;30771140);河南省科技厅国际合作项目(0646630014);河南省教育厅项目(2007180008);河南省国际科技合作重点项目计划资助项目(094300510044);河南大学自然科学基础研究项目(2008YBZR033)

摘　　要：探讨孕期酒精接触对子鼠(P0-P30)视皮质神经元树突棘和突触的影响及其长时程效应。从妊娠第5天(E5)开始用酒精灌胃直至子鼠出生,建立FAS动物模型。采用DiI散射的方法标记视皮质锥体细胞树突棘,利用透射电子显微镜观察突触的超微结构。结果显示,孕期酒精暴露能够诱导子鼠视皮质锥体细胞树突棘的密度降低、长度增加;同时突触超微结构也出现一定的改变(如突触小泡减少、突触间隙变窄和突触后致密区增厚等)。酒精暴露可导致树突棘和突触超微结构的改变,该变化具有剂量依赖性和长时程效应。The prenatal ethanol exposure induced the alterations of dendritic spine and synapse in visual cortex and their long-term effect would be investigated in mice from P0 to P30.Pregnant mice were intubated ethanol daily from E5 through the pup＇s birth to establish mode of prenatal alcohol abuse.The dendritic spines of pyramidal cells in visual cortex of pups were labeled with DiI diolistic assay,and the synaptic ultrastructure was observed under transmission electron microscope.Prenatal alcohol exposure was associated with a significant decrease in the number of dendritic spines of pyramidal neurons in the visual cortex and an increase in their mean length;ultrastructural changes were also observed,with decreased numbers of synaptic vesicles,narrowing of the synaptic cleft and thickening of the postsynaptic density compared to controls.Prenatal alcohol exposure is associated with long-term changes in dendritic spines and synaptic ultrastructure.The changes were dosedependent with long term effect even at postnatal 30.

关 键 词：树突棘 突触 视皮质 透射电子显微镜 DiI散射 

分 类 号：R749.6[医药卫生—神经病学与精神病学]