大鼠骨关节炎三种动物模型的建立及比较  

Establishment and Comparison of Three Types of Osteoarthritis in Mature Rats

作　　者：王婧[1] 张忠辉[1] 孙娇梦[1] 许传营[2] 俞雁[2] 韩伟[1] 

机构地区：[1]上海交通大学药学院再生组学实验室,上海200240 [2]上海交通大学农业与生物学院兽医生物技术重点实验室,上海200240

出　　处：《中国细胞生物学学报》2010年第3期456-460,共5页Chinese Journal of Cell Biology

摘　　要：为探讨三种骨关节炎动物模型的建立方法、效果及其适用条件,采用Hulth手术、碘乙酸关节腔注射、前交叉韧带切除处理,对SD大鼠造骨关节炎模型,并在适当时间,取两侧膝关节进行切片,比较各组骨关节大体形态及病理变化。实验结果表明,Hulth模型组及碘乙酸模型组6周即可造模成功,损伤程度较严重,类似骨关节炎中晚期;前交叉韧带切除模型组造模需8周以上,病情类似骨关节炎早期或中期,更适合用作发病机制、药物治疗方面的研究。The aim of this study was to establish three types of osteoarthritis animal model in Sprague- Dawley mature rats, and deterrnin the effects and applications of three types of osteoarthritis animal models. Rats were randomly divided into Hulth model group, Mono-iodoacetate-induced group, anterior ligament resection model group and sham group. After several weeks of post-surgery, articular cartilages were dissected, embedded in paraffm, sectioned, and performed histological evaluation. The results showed that Hulth model and mono-iodoacetateinduced groups were successfully carried out, and osteoarthritis morphology and symptoms were observed at 6 weeks after surgery, which was similar to the advanced stage of osteoarthritis. Osteoarthritis symptoms were also found at more than 8 weeks after surgery in the anterior ligament resection model group, which was similar to the early or mid-stage of osteoarthritis. In conclusion, anterior ligament resection model was more suitable to investigate the development and pathogenesis of osteoarthritis and potential drug candidates research.

关 键 词：骨性关节炎 动物模型 Hulth模型 碘乙酸 前交叉韧带切除 

分 类 号：R-332[医药卫生]