脂多糖对人牙周膜成纤维细胞MMP-1和TIMP-1表达的影响  

Effect of lipopolysaccharide on the expression of MMP-1 and TIMP-1 in human periodontal ligament cells

作　　者：姜艳[1] 罗小良[1] 靳路远[1] 谢晓莉[1] 

机构地区：[1]中南大学湘雅医院口腔科,湖南长沙410008

出　　处：《牙体牙髓牙周病学杂志》2010年第6期321-325,共5页Chinese Journal of Conservative Dentistry

基　　金：湖南省科技厅项目(08FJ3172);中南大学理科发展基金(1773-206001081)

摘　　要：目的:探讨脂多糖(LPS)诱导下人牙周膜成纤维细胞(HPDLC)表达基质金属蛋白酶1(MMP-1)和金属蛋白酶组织抑制剂1(TIMP-1)的变化。方法:将不同浓度LPS(0.1、1、10、50、100μg/mL)作用于人牙周膜成纤维细胞24h和48h后,运用酶联免疫吸附试验(ELISA)检测上清液中MMP-1和TIMP-1量的变化,并计算MMP-1与TIMP-1的比值。结果:LPS导致HPDLC表达MMP-1显著增强,并呈时间及浓度依赖性;LPS作用24h后可抑制HPDLC的TIMP-1表达,48h时仅100μg/mLLPS有明显抑制作用;MMP-1/TIMP-1比值明显增加。结论:脂多糖具有促进HPDLC分泌基质金属蛋白酶-1的作用,并抑制TIMP-1的表达。AIM： To investigate the effects of lipopolysaccharide （LPS） on matrix metalloproteinase-1 （MMP-1） and tissue inhibitor of matrix metalloproteinase-1 （TIMP-1） induction in human periodontal ligament cells （HPDLCs）. METHODS： HPDLCs were stimulated with different concentrations of LPS （0. 1, 1, 10, 50, 100μg/mL）. After 24 and 48 hours culture, MMP-1 and TIMP-1 levels in the culture media were measured by en- zyme linked immunosorbent assay （ELISA） and the ratio of MMP-1 to TIMP-1 was calculated. RESULTS： LPS en- hanced the expression of MMP-1 in a time- and concentration-dependent manner. TIMP-1 was decreased dose-de- pendently at 24 h post-stimulation, while a significant inhibition of TIMP-1 was observed only with 100 i.Lg/mL LPS treatment at 48h post-stimulation. The ratio of MMP-1 and TIMP-1 was increased significantly. CONCLUSION： LPS can up-regulate MMP-1 expression and down-regulate TIMP-1 expression, therefore may play a role in periapical tissue destruction by breaking the balance of MMP-1/TIMP-1.

关 键 词：脂多糖 基质金属蛋白酶-1 金属蛋白酶组织抑制剂-1 牙周膜成纤维细胞 

分 类 号：R780.2[医药卫生—口腔医学]