AD-p53及联合顺铂治疗卵巢癌裸鼠腹腔移植瘤及腹水的实验研究  

Therapeutic Effects of AD-p53 Combined with Cisplatin to Treat the Intraperitoneal Transplanted Ovarian Tumor and Ascites in Nude Mouse

作　　者：刘婷婷[1] 孔为民[1] 孙立新[2] 张卫华[1] 李静[1] 韩超[1] 

机构地区：[1]首都医科大学附属北京妇产医院,北京100026 [2]中国医学科学院肿瘤医院,北京100000

出　　处：《实用妇产科杂志》2010年第6期429-432,I0001,共5页Journal of Practical Obstetrics and Gynecology

基　　金：北京市卫生局局级基金资助(项目编号:2002-230)

摘　　要：目的:探讨腺病毒载体介导的p53基因(AD-p53)对人卵巢浆液性乳头状腺癌细胞系(SKOV-3)的影响,探讨AD-p53及联合顺铂(DDP)以及不同剂量的AD-p53对卵巢癌裸鼠腹腔移植瘤及腹水的抑制作用。方法:培养SKOV-3细胞,分组后行免疫荧光染色,绘制细胞生长曲线;建立裸鼠腹腔移植瘤模型。共6组:空白对照组、DDP组、低AD-p53组、高AD-p53组、低AD-p53+DDP组、高AD-p53+DDP组。计算抑瘤率及裸鼠腹水形成量。结果:①成功转染基因并表达p53蛋白的细胞在染色后呈红光,当MOI=100及以上时,转染率可达90%以上。②体外实验结果:经AD-p53作用的各组,细胞生长曲线受到不同程度抑制。③体内试验结果:a:各治疗组与空白对照组、联合用药组与单用药组之间瘤重差异均有统计学意义(P﹤0.05),DDP组与单用AD-p53组、不同剂量AD-p53组之间瘤重差异无统计学意义(P>0.05)。b:空白对照组裸鼠均出现血性腹水,量0.4～2.0ml,各治疗组无明显腹水形成。高AD-p53组荷瘤裸鼠的一般状态较其他组差。结论:AD-p53可成功转染SKOV-3细胞,并对细胞生长有抑制作用;AD-p53能抑制卵巢癌裸鼠腹腔移植瘤,并对腹水的形成有抑制作用,其与顺铂有协同抗癌性;无限制地增大AD-p53剂量不会增加疗效,反而会对用药个体不利。Objective：To investigate the effects of AD-p53 on human ovarian cancer SKOV-3 cells and the effects of single use of AD-p53, AD-p53 combined with cisplatin and different doses of AD-p53 on the treatment of intraperitoneal transplanted ovarian tumor and ascites in nude mice. Methods：The human ovarian cancer SKOV-3 cells were cultured, stained by immunofluorescence, and cell growth curve was drawn. Intraperitoneal transplanted ovarian tumor in nude mice were established and divided into six groups： blank control group, DDP group, low AD-p53 group, high AD-p53group, low AD-p53 ＋ DDP group, and high AD- p53 ＋ DDP group. The tumor inhibit rate and the volume of ascites was calculated. Results ：（1） The SKOV-3 cells which transfected genes and expressed p53 protein were stained red. When MOI ≥100, the transfection rate was over 90%. （2） The results of the experiment in vitro showed that the cell growth curve in each group was inhibited in different degree by the role of AD-p53. （3） The results of the experiment in vivo showed that there was statistic difference in the tumor weight among all of the treatment groups and blank control group, single-drug therapy groups and combination therapy groups （ P 〈0.05）. There was no statistic difference in the tumor weight among DDP group and single use of AD-p53 group, different doses of AD-p53 groups（ P〉 0.05）. All of the treatment groups didn＇t have ascited formed while the blank control group had. The volume of ascites was 0.4 - 2.0 ml. The general condition in the high-dose tumor-bearing nude mice was worse than that in the other groups. Conclusions：AD-p53 can be transfected into SKOV-3 cells successfully, and can inhibit cell growth significantly. AD-p53 can inhibit intraperitoneal transplanted ovarian cancer cells growth and ascites formation. AD-p53 combined with DDP has synergetic cancer inhibition effects, however, dose augmentation without limitation won＇t increase its effect besides harmful to the body.

关 键 词：腺病毒科 卵巢癌 裸鼠 肿瘤 顺铂 

分 类 号：R737.31[医药卫生—肿瘤]