葡激酶突变体基因的克隆与表达  

Cloning and Expression of Staphylokinase Mutant Gene

作　　者：叶立文[1] 汤其群[1] 马端 宋后燕[1] 

机构地区：[1]上海医科大学分子遗传学研究室

出　　处：《药物生物技术》1999年第1期9-13,共5页Pharmaceutical Biotechnology

摘　　要：为制备Ｍｒ小的葡激酶以便作口服制剂，用ＰＣＲ技术克隆Ｎ末端缺失１８个氨基酸的葡激酶突变体基因。经大肠杆菌表达，目的蛋白占总菌体蛋白的３２％。表达产物Ｍｒ约为１４０００，在菌体内形成包涵体。经复性后，该突变体对纤溶酶原具有激活作用。Staphylokinase mutant gene,deleted 18 amino acids in N end was amplified from staphylokinase gene,and ligated into pUC19 vector,then recombinated with PLY 4, an expression vector,and transformed E.coli. The expression product of mutant gene was 32% of total E.coli protein,and formed inclusion body.The M r of staphylokinase mutant is 14 000. The mutant has the same immunoreactivity as staphylokinase.

关 键 词：葡激酶 突变体 包涵体 表达 基因克隆 溶栓药 

分 类 号：R973.2[医药卫生—药品] Q78[医药卫生—药学]