p53、PCNA在口腔粘膜癌前病变中的表达及其临床意义  

Expression of p53 protein and PCNA in premalignant lesions of oral mucosa and its clinical significance

作　　者：乐福媛[1] 田臻[1] 张伟国[1] 

机构地区：[1]上海第二医科大学口腔医学院口腔内科教研室

出　　处：《上海口腔医学》1999年第1期18-19,共2页Shanghai Journal of Stomatology

摘　　要：目的观察ｐ５３和ＰＣＮＡ在口腔粘膜癌前病变中的表达特征，进一步探讨其临床意义。方法对５０例临床疑为癌前病变的患者行局部组织活检、光镜检查，作出病理诊断；同时采用免疫组织化学方法进行ｐ５３和ＰＣＮＡ的标记，并将两者结果做比较。结果在癌前病变中，随着上皮细胞的异常增生从基底层向角化层发展，ｐ５３和ＰＣＮＡ阳性表达细胞也随着同步发展。病变伴不同程度异常增生的各病例阳性表达程度之间有显著差异（Ｐ＜０．０１）。结论ｐ５３和ＰＣＮＡ在口腔粘膜癌前病变中的表达呈同步性。且与病变细胞的异常增生程度呈正相关；ｐ５３在癌前病变中的阳性表达可能是其发生癌变的早发事件；癌变的发生可在上皮异常增生的较早期。故ｐ５３和ＰＣＮＡ的检测有利于临床上早期发现有异常增生倾向的病变。Objective The study was to investigate the expression features of p53 protein and PCNA antigens in premalignant lesions of oral mucosa and further to discuss its clinical significance. Methods 50 samples were retrieved from the biopsy of the patients with suspicious premalignancy.Followed by the diagnosis pathologically,the sections were labeled with p53 and PCNA antigens by immunohistochemistry. Results In premalignant lesions,the progression staining with p53 and PCNA antigens were positively corresponding to the epithelial dysplasia from the basal layer to the keratinous layer. Conclusion The p53 and PCNA antigens expressed synchronously in premalignant lesions of oral mucosa and exhibited direct correlation with the degree of epithelial dysplasia;The positive signals with p53 and PCNA antigens in the premalignant lesions may be the earlier incidence in carcinogenesis,which may initiated at the earlier stage of epithelial dysplasia.This phenomenon is deserving of clinicians attention for discovering the premalignant lesions earlier;The detection of p53 and PCNA antigens would be helpful to find out the lesion which has the tendency turning to dysplasia.

关 键 词：P53 PCNA 癌前病变 免疫组化 口腔粘膜肿瘤 

分 类 号：R739.85[医药卫生—肿瘤]