与急性胃黏膜损伤相关体表敏化穴位的动态分布观察  

Distribution of the Activated Acupoints after Acute Gastric Mucosal Injury in the Rat

作　　者：程斌[1] 石宏[2] 吉长福[2] 李江慧[2] 陈淑莉[2] 景向红[2] 

机构地区：[1]山东中医药大学,济南250355 [2]中国中医科学院针灸研究所经络研究中心,北京100700

出　　处：《针刺研究》2010年第3期193-197,共5页Acupuncture Research

基　　金：国家自然科学基金(30672593);北京市自然科学基金(7062049);"973"穴位效应规律研究(2006CB504507)

摘　　要：目的:观察与急性胃黏膜损伤相关的体表伊文思蓝(EB)渗出点的动态分布,并和大鼠穴位的分布进行比较,分析其分布相关性,为疾病状态下穴位敏化的动态过程提供实验依据。方法:Wistar大鼠70只,随机分为正常对照组(10只)、口服稀盐酸组(50只)和口服生理盐水组(10只)。采用空腹口服稀盐酸造成急性胃黏膜损伤模型,当天尾静脉注射EB。分别观察造模当天(口服稀盐酸5h后)以及第2、3、4、5天EB渗出点的分布。结果:急性胃黏膜损伤造成体表一些部位的神经源性炎性反应点,即EB渗出点,这些反应点的分布呈一定的节段性,分布的节段从胸2-腰4(T2-L4),主要分布在胸6-腰1(T6-L1);分布位置主要位于:"膈俞"(相关百分比为47.5%)、"脊中"(相关百分比为58.82%)、"脾俞"(相关百分比为88.23%)、"胃俞"(相关百分比为82.35%)、"中脘"(相关百分比为17.64%)、"上脘"(相关百分比为5.88%)。但这些EB渗出点在正常状态时很少或者不出现,口服稀盐酸组与正常对照组、生理盐水组相比P<0.01,P<0.05。这些渗出点和疾病的过程相关,呈现动态分布,自造模后的2～3d渗出点最多,随着疾病的自愈渗出点消退。结论:急性胃黏膜损伤可促使EB在体表渗出,渗出点呈现神经节段分布并与"脾俞""胃俞"等穴位具有高度相关性,提示在疾病状态下沉寂的穴位可以被激活。Objective To observe the dynamic distribution of the extravasated Evans Blue （EB） dye points （neurogenic inflammatory response） at the skin after acute gastric mucosal injury （AGMI） and its relation to the related regular acupoints in the locations in rats. Methods A total of 70 Wistar rats were randomized into normal control （n = 10）, normal saline （n = 10）, and AGMI （n =50） groups. The AGMI group was further divided into 5 h, 2 d, 3 d, 4 d and 5 d subgroups with 10 rats in each. AGMI model was duplicated by intragastric perfusion of diluted hydrochloric acid （HOl, 0.5 mol/L）. Evans Blue Dye （50 mg/kg, 50 mg/mL in 0.9% saline） was given to the rats before AGMI modeling. The plasma extravasated EB points at the skin of the whole body were observed after removal of the hair. Results The extravasated EB points presented a nerve-segmental distribu- tion, with the proportion of the points in the location being 47.5% for ＂Geshu＂ （BL 17） ,58.82% for ＂Jizhong＂ （GV 6）, 88.23% for ＂Pishu＂ （BL20）, 82.35% for ＂Weishu＂ （BL 21）, 17.64% for ＂Zhongwan＂ （CV 12）, and 5.88% for ＂Shangwan＂ （OV 13）, respectively. The plasma extravasation of EB seldom appeared in normal rats and only fewer points were found in rats accepted administration of 0.9% saline. Significant differences were found between model and normal control groups, and between model and normal saline groups in the numbers of the extravasated EB points （P〈0. 01, P〈0. 05）. The number of the extravasated EB points was related to the phase of gastric mucosa injury, being most on the 2nd and 3re day after modeling and disappearing gradually along with the natural repair of AGMI. Conclusion AGMI promotes the plasma extravasation of EB and the extravasated EB points present a nerve-segmental distribution and have a higher corresponding rate with some acupoints including ＂Pishu＂ （BL 20）, ＂Weishu＂ （BL 21 ）, etc., suggesting an activation of the normally silent acupoints under

关 键 词：急性胃黏膜损伤 伊文思蓝渗出 敏化穴位 神经源性炎性反应 

分 类 号：R224.2[医药卫生—中医基础理论]