人宫颈癌HeLa细胞热疗中的量效与剂量  

Dose-effect Relationship and Equivalent Thermal Dose of Hyperthermia for Cervical Cancer HeLa Cells

作　　者：雷锋林[1] 赵凌云[2,3] 欧阳伟炜[4] 夏启胜[5] 郑双波[6] 马建强[7] 蔡东阳[2,3] 谢小雪[4] 乐恺[6] 王晓文[2,3] 张晓冬[2,3] 高福平[2,3] 唐劲天[2,3] 

机构地区：[1]北京中医药大学生物制药系,北京100102 [2]清华大学工程物理系 [3]粒子技术与辐射成像教育部重点实验室,北京100084 [4]中南大学湘雅医院肿瘤科,长沙410008 [5]中日友好医院临床肿瘤研究所,北京100029 [6]北京科技大学机械工程学院热能工程系,北京100083 [7]北京中医药大学第三附属医院血液肾内科,北京100029

出　　处：《科技导报》2010年第13期73-78,共6页Science & Technology Review

基　　金：国家自然科学基金项目(10775085,30571799)

摘　　要：以人宫颈癌HeLa细胞热损伤为模型,探索新的更简便、准确的热剂量学方法,为临床热疗热剂量的应用提供实验依据。将HeLa细胞经相应温度(37～70℃)和时间(1～30min)的水浴热处理之后,噻唑蓝比色法(MTT法)检测细胞增殖抑制率,流式细胞仪检测细胞凋亡率和坏死率,克隆形成实验观察加热后细胞克隆形成能力。利用热生物学效应的等效关系分析热损伤实验数据,拟合HeLa细胞的热剂量-效应曲线。结果显示,在相同温度下,随着热疗时间的延长,细胞的增殖抑制率增强,存活率降低,克隆形成能力显著降低。在相同时间下,随着热疗温度的增加出现相同的效果。等效热剂量与3种增殖检测方法的实验数据初步吻合,其中克隆形成和流式方法吻合程度较高,MTT法误差较大,不建议作为热疗后细胞增殖检测的常规方法。因此,HeLa细胞在热疗中的量效关系研究对肿瘤热疗实验和临床有一定的参考价值。The dose-effect relationship with respect to the treatment temperatures and heating time for HeLa cells is studied based on a thermal damage model, to obtain a new simpler and quicker thermal dose method. HeLa cells were heated at 37-70℃ in temperaturecontrolled water baths. The tests were divided into nine groups, with each group being further divided into several subgroups according to temperature and time duration （1-30min）. The proliferation inhibition rates were measured by MTT colorimetric assay, the apoptosis rates were determined by flow cytometric analyser, and the colony-forming cells were observed after heating. The experimental cell viability curve was calculated by using the Arrhenius damage model, fitted into the HeLa cells thermal dose-response equivalent curve. Increase of the heating time at the same temperature or increase of the heating temperature at the same heating time would both increase the cell proliferation inhibition rates and reduce the survival rates and the colony forming ability significantly. In addition, a fairly good agreement between experimental data and the equivalent thermal dose is obtained, in which the colony-forming and flow cytometric analyser gave better results, while the MTT colorimetric assay entailed larger error, which was not recommended as a conventional detective method in the detection of cell proliferation after thermotherapy. The hyperthermia dose-effect relationship in HeLa cells will help in the study of tumor hyperthermia experiments and clinical practice.

关 键 词：肿瘤热疗 热剂量 HELA细胞 

分 类 号：R737.33[医药卫生—肿瘤]