定西地区汉族人群HLA-DRB1等位基因多态性研究  

Study on genetic polymorphism of HLA-DRB1 allele in the Hans of Dingxi region

作　　者：李国英[1] 王明禄[1] 刘圆圆[1] 唐朝晖[1] 杨勇毅[1] 李元荣[1] 张继[2] 

机构地区：[1]甘肃省红十字血液中心,甘肃兰州730046 [2]西北师范大学生命科学学院,甘肃兰州730070

出　　处：《西北师范大学学报（自然科学版）》2010年第4期83-86,共4页Journal of Northwest Normal University(Natural Science)

基　　金：中华骨髓库基金资助项目

摘　　要：采用流式反向序列特异性寡核苷酸探针(Flow-rSSO)基因分型技术,对甘肃省定西地区711份健康无血缘关系的汉族人群HLA-DRB1等位基因进行基因分型,研究其分布特点,并与其他地区汉族人群和西北少数民族进行比较.结果表明:711份样本中共检出等位基因13个,等位基因频率较高的是DRB1*12(13.29%)、DRB1*09(12.45%)和DRB1*04(11.95%).其分布特点比较接近北方汉族人群,与裕固族、回族、蒙古族无较大差异,与藏族、维吾尔族存在明显差异,表明其分布具有自身特点.The HLA-DRB1 allele polymorphism distribution of 711 unrelated health Han individuals from Dingxi region in Gansu Province are detected by reverse polymerase chain reaction and sequence-specific olignucleotide probe hybridizations（PCR-SSO）genotyping technology.The results are compared with those of Han populations in China and other minority groups in northwest China.The results show a total of 13 HLA-DRB1 alleles are detected in 711 unrelated health Han individuals,and the most frequent alleles are DRB1＊12（13.29%）,DRB1＊09（12.45%）and DRB1＊04（11.95%）.The HLA-DRB1 allele distribution in Han people of Dingxi has similar characteristics with the northern Han people in China.There is no evident difference between Dingxi population and Yugu,Hui,Mongol population,while evident difference exits between them with Tibetan and Uygur.These show the distribution of Han population from Dingxi region has itself characters.

关 键 词：人类白细胞抗原 流式反向序列特异性寡核苷酸探针 等位基因 多态性 

分 类 号：R392.2[医药卫生—免疫学]