硒多糖、亚砷酸钠对大鼠肝微粒体酶和GSH-Px等的影响  

Effects of selenium polysaccharide and sodium arsenite on rat liver mitocrosomal enzymes and glutathione peroxidase activity

作　　者：程继忠[1,2] 邬惠琼[1,2] 海涛 宋瑞琨[1,2] 

机构地区：[1]同济医科大学环境毒理学教研室 [2]同济医科大学职业医学研究所

出　　处：《卫生毒理学杂志》1999年第1期4-7,共4页Journal of Health Toxicology

基　　金：国家自然科学基金

摘　　要：研究了硒多糖、亚砷酸钠在体内、外对大鼠肝微粒体酶细胞色素Ｐ－４５０、ｂ５、ＮＡＤ（Ｐ）Ｈ－细胞色素Ｃ还原酶、谷胱甘肽硫转移酶（ＧＳＴ）的影响；并通过测定硒多糖、亚砷酸钠对肝谷胱甘肽过氧化物酶（ＧＳＨ－Ｐｘ）和脂质过氧化（ＬＰＯ）的影响，探讨了硒、砷相互作用的机理。结果表明：连续７天腹腔注射０．２ｍｇ／ｋｇ硒多糖，细胞色素Ｐ－４５０、ｂ５的含量、ＧＳＴ的活性降低（Ｐ＜０．０５）；硒多糖明显诱导ＧＳＨ－Ｐｘ的活性，降低脂质过氧化，拮抗亚砷酸钠对ＬＰＯ的作用。亚砷酸钠显著增强肝细胞脂质过氧化（Ｐ＜０．０５）。The effects of selenium polysaccharide and sodium arsenite on the contents of microsomal cytochrome P450,b5 and activities of NAD(P)H cytochrome C reductase and glutathione S transferase in rat were studied in vivo and in vitro experiments.And the mechanism was also illuminated by determining their effects on the activity of glutathione peroxidase(GSH Px) and the lipid peroxidation.The results indicated:the contents of microsomal cytochrome P450 and b5 were decreased, activity of glutathione S transferase was inhibited after seleniunm polysaccharide administered by intraperitoneal injection daily for 7 days at a dose of 0 2mg/kg. The GSH Px activity was increased to 1 7 times of control group and the lipid peroxidation was decreased significantly by selenium polysaccharide( P <0 05).While sodium arsenite induced to increase the content of lipid peroxidation obviously( P <0 05) and had not appear to affect the liver cytosolic glutathione peroxidase and microsomal enzyme activities in vivo.These finding suggested that selenium polysaccharide could protect against lipid peroxidation induced by arsenic. \ \

关 键 词：硒多糖 亚砷酸钠 微粒体酶 GSH-PX 砷中毒 

分 类 号：R135.1[医药卫生—劳动卫生] R977.5[医药卫生—公共卫生与预防医学]