慢性HBV感染者TNFα-308位点多态性与HBV C基因区突变的关系  

Polymorphism of TNFα-308 and mutations of HBV C region in patients with chronic HBV infections

作　　者：原津津[1] 潘晨[1] 黎环[1] 许利军[2] 

机构地区：[1]福州市传染病医院肝病科,350025 [2]肝脏病研究所福州市传染病医院,350025

出　　处：《中华临床感染病杂志》2010年第2期89-92,共4页Chinese Journal of Clinical Infectious Diseases

基　　金：福建省卫生厅青年科研课题资助项目（2006-2-52）;福州市第二批科技计划项目（2007-S-123）

摘　　要：目的 研究慢性乙型肝炎病毒（HBV）感染者肿瘤坏死因子α（TNFα）-308位点的基因多态性与HBV C基因区突变的关系.方法 对95例慢性HBV感染者进行研究,采用聚合酶链反应.限制性片断长度多态性（PCR-RFLP）技术分析患者TNFα-308位点的多态性.对PCR产物直接测序,检测HBV C基因区nt1762/1764、nt1896、nt1899、nt1862、aa60、as87及aa97位点是否存在突变.采用Fisher＇s精确概率法比较TNFα-308位点不同基因型患者HBV C基因区常见突变的检出率.结果 TNFα-308位点共发现3种多态性,分别为G/G型63例（63/95,66.3%）,G/A型28例（28/95,29.5%）,A/A型4例（4/95,4.2%）.TNFα-308位点G/G、G/A、A/A基因型患者HBV C基因区aa87位点及as97位点突变型检出率均分别为39.3%（24/61）、11.5%（3/26）和50.0%（2/4）,差异有统计学意义（F=7.658,P〈0.05）;而nt1762/1764、nt1896、nt1899、nt1862以及aa60位点突变型检出率比较差异无统计学意义（F值分别为0.669、1.542、1.123、2.420和0.966,P值均〉0.05）.结论 慢性HBV感染者TNFα-308位点G/A基因型相对于G/G基因型不易发生HBV抗原性变异,有利于HBV的清除.Objective To investigate the association between polymorphism of TNFα-308 and mutations of HBV C region in patients with chronic HBV infection. Methods Ninety-five patients with chronic HBV infection were recruited in the study. The single nucleotide polymorphism（SNP） of TNFα-308 Was determined by restriction fragment length polymorphism（RFLP）. Mutations of nt1762/1764, nt1896, nt1899, nt1862, as60, aa87 and as97 in HBV C region were detected by direct sequencing after PCR amplification. Mutations of the above points among different genotypes were compared by Fisher＇s exget test. Results Three different genotypes G/G（63/95, 66.3%）, G/A（28/95, 29.5%） and A/A（4/95, 4.2%） were found in TNFα-308 site. The rates of mutations of aa87 and aa97 points in patients with G/G, G/A and A/A genotype were 39.3%（24/61）, 11.5%（3/26） and 50.0% （2/4）, respectively（F=7.658, P〈0.05）;while the mutation rates of nt1762/1764, nt1896, nt1864, nt1899 and aa60 were of no statistical significance among different genotypes（F=0.669, 1.542, 1.123, 2.420 and 0.966, P〉0.05）. Conclusion Compared with G/G genotype, antigenicity of HBV may be more stable in patients with TNFα-308 G/A genotype, which is beneficial to HBV clearance.

关 键 词：肿瘤坏死因子Α 多态性 肝炎病毒 乙型 突变 

分 类 号：R512.62[医药卫生—内科学]