马来眼子菜体内抑藻物质分离及常见脂肪酸抑藻效应  

Isolation of antialgal compounds from Potamogeton malaianus and algal inhibitory effects of common fatty acids

作　　者：胡陈艳[1,2] 葛芳杰[1,2] 张胜花[3] 刘碧云[1] 王静 高云霓[1,2] 吴振斌[1] 

机构地区：[1]中国科学院水生生物研究所淡水生态与生物技术国家重点实验室,武汉430072 [2]中国科学院研究生院,北京100049 [3]云南大学环境与湖泊研究院,昆明650091

出　　处：《湖泊科学》2010年第4期569-576,共8页Journal of Lake Sciences

基　　金：国家自然科学基金项目(30870221;50808172;20877093);国家"十一五"水专项项目(2009ZX07106-002-004);中国博士后科学基金项目(20080431013)资助

摘　　要：提取马来眼子菜植物体内的抑藻物质,分离得到一活性组分,气质联用(GC-MS)鉴定其主要为脂肪酸类物质,抑制羊角月牙藻的半效应浓度(EC50,3d)为7.90mg/L.测定组分中含有的8种脂肪酸与油酸单独及联合作用对羊角月牙藻的抑藻效果,结果证实脂肪酸类物质具有化感抑藻效应,其抑藻效果与其碳链长度及其不饱和度有关,碳链越短其抑藻活性越高,不饱和脂肪酸的抑藻效应大于同碳链饱和脂肪酸,多种脂肪酸联合作用时具有协同作用.分离过程中也得到另一甾醇组分,其对羊角月牙藻没有明显抑制作用,且对脂肪酸类物质的抑藻效果没有影响.One active fraction, which mainly contained many kinds of fatty acids, was extracted and isolated from the submerged maerophyte Potamogeton malaianus, then was analyzed by the GC-MS. The EC50.3d fraction on Selenastrum capricornutum was 7.90mg/L. The inhibition of eight fatty acids in the fraction and oleic acid on S. capricornuturn confirmed that fatty acids had the al- lelopathic inhibitory effect and the effect was relevant to the carbon chain and unsaturated linkages, which meant the shorter the carbon chain of the fatty acid, the stronger the algal growth was inhibited, and the more unsaturated linkages in the fatty acid, the stronger the algal growth was inhibited. The combined activity of multi-fatty acids showed synergistic growth inhibition on S. capricornuturn. Another fraction, which mainly consisted of sterols, was isolated from the extraction of P. rnalaianus, but it did not inhibit the growth of S. capricornutum and had no evident influence on the allelopathic inhibition of fatty acids on S. capricornutum.

关 键 词：马来眼子菜 羊角月牙藻 脂肪酸 化感作用 

分 类 号：Q946[生物学—植物学] X173[环境科学与工程—环境科学]