bFGF单克隆抗体抑制小鼠Lewis肺癌转移及血管新生  

Inhibition of anti-bFGF monoclonal antibody on growth,metastasis and angiogenesis in C57 BL/6 mice Lewis lung carcinoma

作　　者：向军俭[1] 李丹[1,2] 王宏[1] 王盼盼[1] 邓宁[1] 

机构地区：[1]暨南大学生命科学技术学院抗体工程中心,广东广州510632 [2]南方医科大学基础医学院免疫学教研室,广东广州510515

出　　处：《中国癌症杂志》2010年第6期401-405,共5页China Oncology

基　　金：国家"863"专题项目"抑制肿瘤血管新生人源性抗bFGF抗体药物研究"(2009AA02Z112)

摘　　要：背景与目的:碱性成纤维细胞生长因子(basic fibroblast growth factor,bFGF)是一种具有广泛生物学功能的细胞因子,在肺癌等多种肿瘤组织高表达,并参与其发生、发展进程。本研究旨在探讨bFGF单克隆抗体对C57BL/6小鼠Lewis肺癌移植瘤生长、转移及血管新生的抑制作用。方法:采用皮下接种肿瘤细胞的方法,建立Lewis肺癌自发转移小鼠模型,随机分为PBS组、bFGF单克隆抗体MabF7治疗组,以及正常小鼠IgG阴性对照组,每组8只。自接种第9天开始给药,每3天1次,连续6次。同时观察Lewis肺癌小鼠健康状况,游标卡尺测量皮下移植瘤体积,给药6次后处死小鼠,称瘤质量,取肺组织计数各组小鼠肺表面转移瘤结节数,并用免疫组化法检测CD31的表达,以计算肿瘤组织微血管密度(microvessel density,MVD)。结果:MabF7治疗组小鼠肿瘤体积与PBS组相比生长缓慢,小鼠瘤质量为(2.6±1.0)g,较PBS组的(5.1±1.3)g显著降低(P<0.05);MabF7组小鼠肺表面转移瘤结节数为(3.0±2.1)个,显著低于PBS组的(12.3±2.4)个(P<0.05)。另外MabF7组可显著抑制肿瘤组织MVD表达水平。结论:bFGF单抗可明显抑制C57BL/6小鼠Lewis肺癌生长及转移,其作用与抑制肿瘤增殖及微血管生成相关。Background and purpose： Basic fibroblast growth factor （bFGF）, acting as a broad-spectrum mitogen, is highly expressed in many tumors such as lung cancer, bFGF also plays as an important role in tumor growth and development. This study was aimed to investigate the inhibitory effects of anti-bFGF monoclonal antibody on the growth, metastasis and angiogenesis in Lewis lung carcinoma. Methods： The mouse lung cancer graft model was established through injection of the Lewis lung carcinoma cells subcutaneously into the right flank of C57BL/6 mice. When palpable tumors （≥5 mm in diameter） developed, anti-bFGF mAbs in 200 μL PBS were injected subcutaneously around the tumors. Control mice received equal amounts of control IgG or PBS. Tumor volume and metastases of Lewis lung carcinoma were later counted. The microvessel density （MVD） in the tumor mass was measured by staining for endothelial cell surface molecules such as CD31 by using immunohistochemistry. Results： Anti-bFGF mAb could inhibit tumor growth in C57BL/6 mice Lewis lung cancer model. Tumor weight in MabF7 group was （2.6±1.0） g, which was lower than that in the PBS group [（5.1±1.3） g] （P〈0.05）. The mean number of metastatic lung nodules in MabF7 group was 3.0±2.1, which was lower than that of PBS group （12.3±2.4） （P〈0.05）. Moreover, MabF7 reduced the expression of MVD in the tumor tissue. Conclusion： Anti-bFGF mAb could inhibit tumor growth and pulmonary metastasis in C57BL/6 mice Lewis lung cancer model and could suppress angiogenesis of tumor tissue.

关 键 词：BFGF 单克隆抗体 肿瘤转移 微血管密度 

分 类 号：R730.2[医药卫生—肿瘤]