Bcl-2和Fas在人食管癌细胞Ec-9706细胞中表达及中药干预作用  

THE GENE EXPRESSION OF BCL-2 AND FAS IN HUMAN ESOPHAGEAL EPITHELIA EC-9706 CELLS AND INTERFERENCE OF CHINESE MEDICINE

作　　者：杨旭东[1] 张杰[1] 吴英俊[1] 张春军[1] 董凯[1] 

机构地区：[1]牡丹江医学院,黑龙江牡丹江157011

出　　处：《牡丹江医学院学报》2010年第3期1-2,共2页Journal of Mudanjiang Medical University

基　　金：黑龙江省卫生厅科研项目(No.2007-003)

摘　　要：目的:研究白毛藤诱导人食管癌细胞凋亡的作用及其机制。方法:荧光显微镜观察不同浓度白毛藤诱导Ec-9706细胞凋亡的作用,RT-PCR检测Bcl-2、Fas基因表达量的变化。结果:白毛藤具有诱导Ec-9706细胞凋亡的作用,并且上调Fas基因、降低Bcl-2基因的表达。结论:白毛藤促进Ec-9706细胞凋亡,其机制可能与激活Fas基因、抑制Bcl-2基因表达有关。Objective：To study the effect of Solamum lyratum Thunb on apoptosis of Ec-9706 cells and its mechanism.Methods：Ec-9706 cells were treated with various doses of Solamum lyratum Thunb.We observed apoptosis of Ec-9706 cells by fluorescence microscope.We detected the levels of Bcl-2 and Fas gene expression in all groups by RT-PCR.Results： Solamum lyratum Thunb has obviously apoptosis-inducing effects on Ec-9706 cells and could effectively decrease the level of Bcl-2 gene expression and improve the level of Fas gene expression.Conclusion： Solamum lyratum Thunb could induce apoptosis of Ec-9706 cells probably through down regulating Bcl-2 gene expression and up regulating Fas gene expression.

关 键 词：BCL-2基因 FAS基因 细胞凋亡 白毛藤 

分 类 号：R73[医药卫生—肿瘤]