凋亡抑制蛋白PED/PEA-15和XIAP在肝细胞肝癌中的表达及意义  

Expression and clinical significance of anti-apoptotic factors PED/PEA-15 and XIAP in hepatocellular carcinoma

作　　者：孙德光[1] 吴益峰[1] 高振明[1] 梁锐[1] 王立明[1] 

机构地区：[1]大连医科大学附属第二医院普外科,116027

出　　处：《中华医学杂志》2010年第24期1660-1664,共5页National Medical Journal of China

基　　金：国家“863”高技术研究发展计划（2006AA02A309） 志谢 本研究得到了中国科学院大连化学物理研究所邹汉法研究员和叶明亮研究员的悉心指导和无私帮助,在此一并表示诚挚的谢意

摘　　要：目的 探讨凋亡抑制蛋白PED/PEA-15和XIAP在肝细胞肝癌中的表达及临床病理因素的关系.方法 我们应用RT-PCR和Western印迹检测了40例肝细胞肝癌和相应癌旁组织及12例正常肝组织中PED/PEA-15和XIAP mRNA及蛋白的表达,并结合临床病理特征进行分析.结果 肝细胞肝癌组织中PED/PEA-15和XIAP mRNA蛋白的表达均明显高于癌旁组织和正常肝组织（相对吸光度比值为0.636±0.061、0.352±0.068、0.179±0.036与0.579±0.090、0.344±0.084、0.184±0.038）（P均＜0.01）,PED/PEA-15和XIAP mRNA和蛋白的表达在不同年龄、性别、肿瘤大小、肿瘤数目、门静脉转移和肿瘤复发间的差异无统计学意义（P均＞0.05）,在不同的病理分级和临床分期间差异有统计学意义（P＜0.05）.结论 凋亡抑制蛋白PED/PEA-15和XIAP在肝细胞肝癌中呈高表达,可作为判断人肝细胞肝癌生物学行为的参考指标且有望成为肝细胞肝癌靶向基因治疗的新靶点.Objective To investigate the expression of apoptosis arrestin PED/PEA-15 and XIAP in hepatocellular carcinoma （ HCC） and explore their relationship with clinicopathological factors of HCC.Methods The mRNA expressions of PED/PEA-15 and XIAP genes were detected by RT-PCR （ reverse transcription-polymerase chain reaction） in resected liver tumor tissues and corresponding adjacent noncancerous tissues from 40 HCC patients and normal liver tissues from 12 patients with benign lesions.Their protein levels were detected by Western blot. Results The mRNA expressions of PED/PEA-15 and XIAP genes and their proteins were significantly higher than those in adjacent tissues and normal tissues （0.636±0.061±0.352±0.068±0.179 ±0. 036;0. 579 ±0.090±0.344 ±0.084±0.184±0.038） （P 〈0. 01）. The mRNA levels of PED/PEA-15 and XIAP genes and their protein levels were significantly associated with the pathological grade and clinical stage of HCC （P 〈 0. 05）. However there was not a significant correlation with such clinicopathological features as age, gender, size of tumor, tumor load,metastasis and recurrence （P 〉 0. 05）. Conclusion The expressions of apoptosis arrestin PED/PEA-15 and XIAP are elevated in HCC as compared with adjacent tissues and normal tissues. Thus it may serve as both a reference indicator for biological behaviors of HCC and a new target for gene therapy.

关 键 词：肝细胞癌 PED/PEA-15 XIAP RT-PCR 

分 类 号：R735.7[医药卫生—肿瘤]