基因型对陆地棉花药离体培养反应的影响  

Effect of Genotype on Cotton Anther Response in in vitro Culture

作　　者：张宝红[1] 李凤莲[1] 李秀兰[1] 李付广[1] 刘方[1] 姚长兵[1] 刘传亮[1] 

机构地区：[1]中国农业科学院棉花研究所

出　　处：《棉花学报》1999年第2期92-99,共8页Cotton Science

基　　金：国家自然科学基金

摘　　要：对２７种棉花基因型的花药在离体培养中的反应进行了比较，结果表明：所有的基因型在合适的培养条件下均能高频率地形成愈伤组织，但不同基因型间愈伤组织诱导率和诱导量不同；再生植株的花药比原始亲本的花药易诱导出愈伤组织；品种间杂种表现出部分优势，但不明显。本试验中，仅鲁棉６号和Ｓｉｏｋｒａ１－３两个品种诱导获得了胚性愈伤组织、胚状体和再生植株，且二者之间也存在着差别。从基因型在棉花花药培养中的效应、外界调控对棉花不同基因型花药培养效果的影响、棉花花药培养与体细胞培养基因型差异的比较等三个方面进行了讨论，提出了棉花花药培养愈伤组织诱导和植株再生是受两套遗传基因控制的遗传性状。Twenty-seven genotypes of Gossypium hirsutum L. were comparatively examined with regard to their response in in vitro culture. All of genotypes could obtained high frequency callus in suitable medium, but the induction rate and growth of callus were various among genotypes, the anther of regenerative plants was easier induced than that of parents; a little superiority appeared in the anther culture of hybrid than two parent cultivars. In this experiment, embryogenic callus, embryoids and regenerative plants were produced only from anther callus of Lumian No.6 and Siokral 1-3,and difference existed between the two cultivars. This paper discussed the following three questions: effects of genotypes on cotton anther culture, effects of outside regulation and control on the effects of various genotypes on cotton anther culture, and comparison of genotypes difference between somatic cell culture and anther culture of cotton; put forward that the callus induction and plant regeneration were mutulstand alone genetic characters that were respectively controlled by two set of genes, and put forward the threshold of cotton tissue culture and plant regeneration.

关 键 词：陆地棉 花药培养 基因型 植株再生 基因效应 

分 类 号：S562.01[农业科学—作物学]