气相色谱-质谱联用法检测新生儿脐带血多环芳烃  

Determination of Polycyclic Aromatic Hydrocarbons in Newborn Umbilical Cord Blood by Gas Chromatography-mass Spectrometry

作　　者：郭勇勇[1] 霍霞[1] 吴库生[1] 陈爱明[2] 彭琳[1] 班皓[1] 刘俊晓[1] 徐锡金[1] 

机构地区：[1]汕头大学医学院分析细胞学实验室,广东汕头515041 [2]美国辛辛那提大学医学院环境健康中心流行病学与生物统计学研究室

出　　处：《汕头大学医学院学报》2010年第2期68-70,共3页Journal of Shantou University Medical College

基　　金：国家自然科学基金资助项目(30972513)

摘　　要：目的:建立新生儿脐带血中16种多环芳烃(PAHs)的分析方法。方法:脐带血经NaOH-乙醇水溶液皂化后,用正己烷萃取;萃取的有机相经SPE柱净化后,用气相色谱-电子电离源-质谱联用仪,选用离子检测法和外标法进行定性和定量。结果:加标回收率73.0%～112.0%;样品回收率81.6%;方法检出限0.03～0.11ng。结论:该方法检测电子垃圾拆解区新生儿脐带血中16种PAHs,灵敏度高、重现性好、回收率良好。Objective：To develop a method for determination of polycyclic aromatic hydrocarbons（PAHs）in newborn umbilical cord blood by gas chromatography-mass spectrometry（GC-MS）.Methods：The umbilical cord blood sample saponified with NaOH in EtOH-H2O at 60 ℃ for 30 minutes,was extracted with n-hexane in amber colored,capped conical flask.Cleanup of the extract was performed by SPE cartridges ahead of GC-MS analysis with electronic ionization in selective ion monitoring mode.Results：The recovery of the standard addition was 73.0%～112.0%.The recovery of the samples was 81.6%.The detection limit of the method was in the range of 0.03～0.11 ng.Conclusion：The method developed for determination of 16 PAHs in newborn umbilical cord blood from an e-waste recycling area is sensitive,high reproducible with satisfied recoveries.

关 键 词：多环芳烃 脐带血 新生儿 气相色谱-质谱 电子电离源 

分 类 号：R917[医药卫生—药物分析学]