动态浊度法在血站细菌内毒素检测中的应用  

Implementation of Dynamic Turbidometric Assay for the Testing of Bacterial Endotoxin in the Blood Center

作　　者：古醒辉[1] 马兰[1] 尚桂芳[1] 刘怡[1] 何柳媚[1] 邬旭群[1] 

机构地区：[1]深圳市血液中心,深圳518035

出　　处：《热带医学杂志》2010年第6期678-680,共3页Journal of Tropical Medicine

摘　　要：目的探讨32孔动态浊度试管仪在血站细菌内毒素检测中的应用情况。方法对血站原辅材料进行抽检,使用动态浊度试管仪测定值做标准曲线,并检测其细菌内毒素含量。结果标准曲线显示该方法线性关系好,实验稳定,灵敏度高;原辅材料的细菌内毒素含量均小于药典的规定限,结果合格。结论 32孔动态浊度试管仪在血站原辅材料细菌内毒素检测中的应用良好,使用的原辅材料质量优良。Objective To investigate the use of 32-Well Tube Reader in the testing of bacterial endotoxin in blood centers.Methods Raw and subsidiary materials were randomly sampled and analyzed for bacterial endotoxin using the 32-Well Tube Reader.Linearity,reproducibility and the sensitivity were determined from the standard curves.Results The tube reader was stable and sensitive in the determination of endotoxin.The levels of endotoxin in the samples were well below the required limit.Conclusion The 32-Well Tube Reader is suitable for the testing of endotoxin in the blood centers.

关 键 词：浊度仪 原辅材料 内毒素检测 

分 类 号：R446.119[医药卫生—诊断学]