金霉素链霉菌发酵培养基的优化  

Optimization of the Fermentation Medium with Streptomyces aureofaciens

作　　者：高山[1] 李宁环[1] 胡平[2] 何义发[1] 

机构地区：[1]湖北民族学院生物科学与技术学院,湖北恩施445000 [2]恩施职业技术学院生物工程系,湖北恩施445000

出　　处：《湖北民族学院学报（自然科学版）》2010年第2期211-215,共5页Journal of Hubei Minzu University(Natural Science Edition)

基　　金：湖北省教育厅科学技术研究重点项目(D20092904)

摘　　要：金霉素链霉菌能产生四环素族抗生素,而抗生素是微生物的次级代谢产物,其产量与培养基的组成成份及培养条件密切相关.通过单因素试验考察金霉素链霉菌生长所需要的碳源和氮源,在此基础之上再通过4因素3水平正交试验对金霉素链霉菌发酵培养基进行优化筛选,其4因素分别为玉米淀粉、黄豆粉、硫酸铵、酵母粉的质量分数.试验结果表明,优化后得到的最佳培养基,用管碟法测得的试验菌种发酵液的生物效价达到183.2μg/mL.对正交结果进行极差分析得知,发酵培养基中酵母粉质量分数对试验菌种发酵产抗生素影响最大,其次为玉米淀粉,再次为黄豆粉,硫酸铵对抗生素产量影响最小.筛选得到的最佳发酵培养基组成为:玉米淀粉100.0 g/L,黄豆粉50.0 g/L,硫酸铵6.0 g/L,酵母粉4.0 g/L.Streptomyces aureofaciens produce tetracyclines,while antibiotics are secondary metabolites of microorganisms.The yield is closely related to the composition of the medium and culture conditions.This article first investigated the carbon and nitrogen sources by single factor experiments which are necessary to the growth of S.aureofaciens.On this basis,orthogonal design（L9（34））was adopted to optimize the fermentation medium of S.aureofaciens.Its four factors were corn starch,soybean flour concentration and ammonium sulfate concentration,yeast extract.Experimental results show that biological effect of the best medium optimized by using the experimental measured cylinder-plate method fermentation of liquid in the tested strains is 183.2 μg/mL.Orthogonal range analysis of the results indicates that,in yeast fermentation medium,the mass fraction of the yeast extract affects the yield of antibiotics most,followed by corn starch and the concentration of soybean powder,ammonium sulfate on the production of antibiotics in the least.Selection to the best culture medium composition： corn starch 100.0 g/L,soy flour 50.0 g/L,ammonium sulfate 6.0 g/L,yeast extract 4.0 g/L.

关 键 词：金霉素链霉菌 抗生素 发酵 培养基优化 管碟法 

分 类 号：Q939.9[生物学—微生物学]