蔬菜中甲胺磷等5种有机磷农药残留量的分散液-液微萃取/气质联用技术检测  

Determination of Five Organophosphorus Pesticide Residues in Vegetables Based on Dispersive Liquid-Liquid Microextraction Coupled with Gas Chromatography-Mass Spectrometry

作　　者：杜晓婷[1] 周敏[1] 张剑[2] 曾延波[2] 翟云云[2] 李蕾[2] 

机构地区：[1]杭州市质量技术监督检测院,浙江杭州310014 [2]嘉兴学院生物与化学工程学院,浙江嘉兴314001

出　　处：《分析测试学报》2010年第7期751-754,共4页Journal of Instrumental Analysis

基　　金：浙江省科技计划资助项目(2007C23071;2007F70011);浙江省自然科学基金资助项目(Y40802520);嘉兴市科技计划资助项目(2008AY2016)

摘　　要：建立了分散液-液微萃取/气相色谱质谱（DLLME/GC-MS）联用技术分析蔬菜样品中甲胺磷、甲拌磷、甲基对硫磷、毒死蜱和乐果5种有机磷农药残留的新方法。优化后的萃取条件：10μL氯苯为萃取剂,1.0 mL丙酮为分散剂,萃取时间为3 m in。5种有机磷农药均具有良好的线性关系,相关系数不低于0.995,加标回收率为60%~95%,相对标准偏差为2.8%~9.1%,检出限为0.001~0.140 mg/kg。应用于蔬菜中有机磷农药残留的检测,结果满意。A new method was developed for the determination of 5 organophosphorus pesticide residues,including methamidophos,phorate,methyl parathion,chlorpyrifos,dimethoate,in vegetables by dispersive liquid-liquid microextraction（DLLME） coupled with gas chromatography-mass spectrometry（GC-MS）.The extraction conditions were optimized.A good extraction efficiency was obtained by using 10 μL chlorobenzene as extraction solvent,0.8 mL acetone as disperser solvent extracting for 3 min.The calibration curves for 5 organophosphorus pesticides all had good linear relationships with correlation coefficients not less than 0.995.The spiked recoveries ranged from 60% to 95% with relative standard deviations of 2.8%-9.1%.The limits of detection were in the range of 0.001-0.140 mg/kg.The method was applied in the determination for organophosphorus pesticide residues in vegetables with satisfactory results.

关 键 词：分散液相微萃取 有机磷农药 气相色谱质谱联用 蔬菜 

分 类 号：O657.63[理学—分析化学] F767.2[理学—化学]