碳氮源对复合木质素降解菌木质素降解能力及相关酶活的影响  

Effect of Carbon and Nitrogen Sources on Lignin Degradation Ability and Enzyme Activity of Dual-Cultured,Lignin-Degrading Fungi

作　　者：范寰[1] 梁军锋[2] 赵润[2] 张金凤[2] 张洪生[2] 

机构地区：[1]天津市畜牧兽医研究所,天津300112 [2]农业部环境保护科研监测所资源再生研究室,天津300191

出　　处：《农业环境科学学报》2010年第7期1394-1398,共5页Journal of Agro-Environment Science

基　　金：农业部产地环境与农产品安全重点开放实验室资助

摘　　要：白腐真菌所具有的降解木质素能力源于其所产生的酶系统,碳源和氮源是其降解木质素和产酶的一个极为重要的影响因素。通过室内小麦秸秆固态发酵试验,研究了不同的碳、氮源对两株侧耳属真菌Tf1(P.pulmonarius)和JG1(P.cornucopiae)产酶活力、木质素降解和粗蛋白含量的影响。结果表明,Lip和MnP是参与复合木质素降解菌Tf1+JG1降解小麦秸秆重要的木质素降解酶。以葡萄糖为碳源,酒石酸铵为氮源能显著提高复合木质素降解菌对木质素的降解能力,发酵9 d后小麦秸秆的失重率为14.87%,木质素含量为8.68%,木质素降解率为22.95%;粗蛋白含量为7.28%,比未发酵麦秸提高了36.84%(P<0.05);Lip和MnP活力分别为629.11 U.g-1和622.22 U.g-1。White-rot fungi are characterized by their lignin-degrading capability derived from their ligninolytic enzyme system.Carbon and nitrogen sources are important factors for the lignin degradation rate and enzyme activity of these fungi.The present study was conducted to investigate the effect of selected carbon and nitrogen sources on lignin degradation ability,ligninolytic enzyme activity and crude protein con-tents by dual culture of two Pleurotus strains（P.pulmonarius Tf1 and P.cornucopiae JG1）on wheat straw substrates.Lignin peroxidase（Lip） and manganese-dependent peroxidase（MnP）were the major lignin-degrading enzymes for the dual-cultured fungi Tf1＋JG1.Carbon source（glucose,cane sugar,maizena,maltose and starch）and nitrogen source（ammonium tartrate,ammonium sulfate,peptone,wheat bran,soybean meal）were tested in these experiments.The greatest lignin degradation ability of Tf1＋JG1 was found using glucose as carbon source and am-monium tartrate as a nitrogen source.After fermentation for 9 days,weight loss for wheat straw was 14.87%,lignin content was 8.68% and lignin degradation was 22.95%.The crude protein content was 7.28%,which is 36.84% greater than non-fermented wheat straw.The Lip and MnP activities were 629.11 U.g-1and 622.22 U.g-1,respectively.

关 键 词：碳源 氮源 木质素降解菌 木质素过氧化物酶 锰过氧化物酶 漆酶 

分 类 号：X172[环境科学与工程—环境科学]