马铃薯凝集素的制备  

Extraction and purification of the lectin from potato tubers

作　　者：华绍烽[1] 李广林[1] 陈吉祥[1] 赵青云[1] 宋秉生[1] 

机构地区：[1]中国农业科学院兰州畜牧与兽药研究所,兰州730050

出　　处：《西北药学杂志》1999年第1期7-9,共3页Northwest Pharmaceutical Journal

基　　金：甘肃省自然科学基金!ZR-96-047

摘　　要：甘肃马铃薯（2．2kg）经缓冲液提取、硫酸铵分级沉淀、DEAE－纤维素、CM－纤维素离子交换、SephadexG150，Senharose6B葡聚糖凝胶层析纯化、水透析和冻干后制得凝集素，总活力为495120u，比活为6641．89u／mg；经聚丙烯改胺凝胶电泳证明是均一的；氨基酸分析表明富含天冬氨酸、谷氨酸、亮氨酸和赖氨酸。The extraction and purification of potato lectin (2. 2 kg) were described. The acetic saltbuffer was used as extracting solvent. The extract was chromatographied with DEAE-cellulose column,CM-cellulose column, G-150 dextrane gel and 6B dextrane gel. The precipitation was dialysed in water andfreeze-dried. The general activity of the potato lectin is 491520 units and specitic activity is 6641. 89 unitsper milligram. The potato lectin was homogeneous indentified by polyamide gel electrophoresis. Asparticacid is the most abundant aminoacid in potato lectin.

关 键 词：马铃薯凝集素 提纯 层析 氨基酸 制备 

分 类 号：R392－33[医药卫生—免疫学]