黑龙江省满山红油的GC-MS分析  

GC-MS Analysis of Components in Oleum Rhododendri Daurici from Heilongjiang Province

作　　者：方洪壮[1] 孙国东[1] 宗希明[1] 孙长海[1] 

机构地区：[1]佳木斯大学药学院,黑龙江佳木斯154007

出　　处：《中国实验方剂学杂志》2010年第8期47-51,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：黑龙江省中医管理局重点项目(ZHY06-Z43)

摘　　要：目的:解析并比较黑龙江省不同地区的满山红油的化学成分。方法:采用水蒸气蒸馏法提取挥发油。用GC-MS法对完全分离的色谱峰定性,用化学计量学HELP方法解析重叠峰。以色谱峰面积归一化法获得各化合物的相对含量,并进行主成分分析。结果:共鉴定85种成分,其中共有成分32种。15个样品按其化学成分可分为4类。6个满山红油中未检出杜鹃酮。结论:GC-MS法结合化学计量学方法分析满山红油,可获得更多的信息。Objective：To analyze and compare the chemical components of oleum rhododendri daurici from different regions of Heilongjiang province.Method：The volatile oils were extracted by steam distillation.Chromatographic peak of complete separation were analyzed by capillary gas chromatography-mass spectrometry （GC-MS） and overlapping chromatographic peaks analyzed by heuristic evolving latent projections （ HELP） of chemometrics.The relative content of each component in volatile oil was calculated by normalization of peak area, and the method of principal component analysis is taken to analyze the data of qualitative and quantitative.Result：A total of 85 components were identified,of which 32 compounds were existed in all the samples.According to chemical components,15 samples were divided into four categories.No germacrone was detected in 6 samples.Conclusion：More information can be obtained from oleum rhododendri daurici,combined GC-MS analysis with chemometric method.

关 键 词：满山红油 气相色谱-质谱 化学计量学 

分 类 号：R284.1[医药卫生—中药学]