异氟醚对幼兔缺血再灌注心肌细胞凋亡及Bcl-2、Bax和Caspase-3表达的影响  

Effects of isoflurane on apoptosis of myocardial cells and the expression of Bcl-2 Bax and caspase-3 after ischemia-reperfusion in immature rabbits

作　　者：方衍锋[1] 刘国利[1] 张锦英[1] 

机构地区：[1]辽宁医学院附属第一医院麻醉科,辽宁锦州121001

出　　处：《中国现代医学杂志》2010年第12期1797-1801,共5页China Journal of Modern Medicine

基　　金：辽宁省高等学校科研项目(No:2008400)

摘　　要：目的探讨异氟醚对幼兔缺血再灌注心肌细胞凋亡及Bcl-2、Bax和Caspase-3表达的影响。方法取32只日本大耳白幼兔随机分为4组,假手术组:冠状动脉(冠脉)只穿线不结扎;缺血再灌注组:结扎冠脉30min,再灌注2h;异氟醚预处理组:缺血前吸入1.1%异氟醚30min,洗脱15min后处理同缺血再灌注组;格列苯脲组:异氟醚吸入前于耳缘静脉注射格列苯脲0.5mg/kg后处理同异氟醚预处理组。观察心肌细胞凋亡和Bcl-2、Bax和Caspase-3蛋白表达情况,电镜观察超微结构。结果异氟醚预处理组细胞凋亡较缺血再灌注组和格列苯脲组明显减少(P<0.05),异氟醚预处理组Bcl-2表达高于缺血再灌注组和格列苯脲组(P<0.05),而Bax和Caspase-3表达少于缺血再灌注组和格列苯脲组(P<0.05),电镜显示异氟醚预处理组细胞损伤程度小于缺血再灌注组和格列苯脲组。结论异氟醚预处理对幼兔缺血再灌注心肌损伤有明显的保护作用,其机制与促K-ATP通道开放有关。【Objective】To investigate the effects of isoflurane on apoptosis of myocardial cells and expression of Bcl-2、Bax and caspase-3 after ischemia-reperfusion in immature rabbits.【Methods】Thirty-two Japanese Big-ear rabbits were randomly divided into four groups （eight per group）： In the sham group, the heart was exposed, but the coronary artery was not ligated; In the ischemia-reperfusion group： the rabbits were subjected to 30 min. of the left anterior descending coronary artery occlusion followed by a 2-h reperfusion. In the isoflurane preconditioned group： the rabbits were pretreated with isoflurane （1.1%） 30 min. before ischemia. In the Glyburide group： the rabbits were treated with Glyburide （0.5 mg/kg） before breathing in isoflurane. Apoptosis of myocardial cells was detected by TUNEL method, the expression of Bcl-2 Bax and caspase-3 was examined by immunohistochemical technique, and the fine structure was observed under electron microscope as well. 【Results】The apoptosis of myocardial cells in isoflurane group and sham operation group were significantly less than that of ishemia -reperfusion group and Glyburide group （P〈0.05）. The expression of Bcl -2 was significantly higher in isoflurane group than that of ischemia-reperfusion and Glyburide group （P〈0.05）, but the contents of Bax and caspase-3 were significantly lower in ischemia-reperfusion and Glyburide group （P〈0.05）. The injury extent of isoflurane group was smaller than that of ischemia -reperfusion and Glyburide group based on the changes of the cellular structure under electronmicroscope. 【Conclusions】Isoflurane can decrease apoptosis of myocardial cells induced by ischemia-reperfusion. Isoflurane-induced preconditioning may provide significant myocardial protection against ischima-reperfusion injury in immature rabbit hearts and K-ATP channels is likely involved in this protective mechanism.

关 键 词：异氟醚 缺血再灌注 细胞凋亡 

分 类 号：R-332[医药卫生] R364.4