基于分子信标定量检测肿瘤细胞p21 mRNA表达变化  

Quantitative Detection of p21 mRNA in Tumor Cells Based on Molecular Beacon

作　　者：刘斌[1,2,3] 羊小海[1,3] 王柯敏[1,2,3] 谭蔚泓[1,2,3] 

机构地区：[1]化学生物传感与计量学国家重点实验室湖南大学化学化工学院,长沙410082 [2]湖南大学生命科学与技术研究院,长沙410082 [3]生物纳米与分子工程湖南省重点实验室,长沙410082

出　　处：《化学学报》2010年第13期1303-1307,共5页Acta Chimica Sinica

基　　金：国家自然科学基金(No.90606003);湖南省杰出青年科学基金(No.08JJ1002)资助项目

摘　　要：根据p21基因序列设计合成特异性检测p21mRNA的分子信标,发展了一种体外快速、定量检测p21mRNA的新方法,将其用于肿瘤细胞p21mRNA表达水平的检测.结果发现:抑制p53表达引起CNE2细胞中p21mRNA表达水平降低,转染ING1诱导MCF-7细胞中p21mRNA表达上调,5-氟尿嘧啶处理MCF-7细胞后p21mRNA表达水平呈浓度和时间相关性变化.这种特异性强、操作快速、简便的新方法有望广泛用于各类样品中p21mRNA表达水平检测.A molecular beacon,which was designed and synthesized according to the p21 gene sequence,was applied to develop a new method for quantitative detection of p21 mRNA in cellular total RNA.The results showed that p21 mRNA is inhibited by silencing p53 in CNE2 cells while it is upregulated in MCF-7 cells by ING1 transfection,the influence of 5-FU treatment on p21 mRNA of MCF-7 cells is time and concentration-dependent manner.These results demonstrated that the new method with the advantage of high specificity,fast speed and simplicity can be widely applied for p21 mRNA detection in different samples.

关 键 词：分子信标 P21 RT-PCR 定量检测 

分 类 号：R730.4[医药卫生—肿瘤]