TLR4在人胆囊癌细胞株SGC-996的表达及对裸鼠移植瘤生长的影响  

TLR4 expression in human gallbladder carcinoma cell Line SGC-996 and affects growth of xenograft tumor in nude mice

作　　者：彭欢[1] 胡志前[1] 唐茂盛[1] 

机构地区：[1]第二军医大学长征医院普通外科,上海200003

出　　处：《中华实验外科杂志》2010年第7期860-862,共3页Chinese Journal of Experimental Surgery

摘　　要：目的 观察Toll样受体4（TLR4）在人胆囊癌细胞SGC-996的表达及RNA干扰TLR4基因表达后对裸鼠体内成瘤能力的影响.方法 采用免疫组化及逆转录.聚合酶链反应（RT-PCR）检测TLR4在SGC-996细胞的表达.将12只BALB/C裸鼠随机分为两组,实验组皮下注射6×106个经TLR4小干扰RNA稳定转染后的SGC996细胞,对照组注射同数量未经处理的SGC996细胞,连续观察5周,比较两组肿瘤的生长情况,计算成瘤率、生长体积和生长率.结果 SGC-996细胞阳性表达TLR4.两组裸鼠成瘤率均为100%,瘤体平均体积分别为（277.94±7.60）mm3和（474.47±9.99）mm3,平均生长率分别为7.941%和13.556%,瘤体质量分别为（0.76±0.16）g和（1.78±0.23）g,差异有统计学意义（P〈0.05）.结论 TLR4表达于人胆囊癌细胞SGC-996,小干扰RNA转染后可以降低TLR4基因表达,从而使SGC996细胞体内成瘤能力下降.Objective To observe the expression of TLR4 in human gallbladder cancer cell line SGC-996,and investigate whether RNA interference targeting TLR4 gene affect growth of the gallbladder carcinoma xenograft in nude mice. Methods The expression of TLR4 in SGC-996 was determined bv im-munohistochemistry and reverse transcription-polymerase chain reaction （ RT-PCR）. 12 BALA/C nude mice were randomly divided into two groups. The experimental group was injected 6 x 10 SGC996 cells stable transfected by small interfering RNA for TLR4 per mice. The control group was injected with the same number of untreated SGC996 cells. Aftter continuous observation for 5 weeks, the formation rate, growth rate, weight and volume of the tumors were measured and calculated. Results The results of immunohistochem-istry and RT-PCR indicated the expression of TLR4 in SGC-996. The tumor formation rate of gallbladder carcinoma cell and TLR4-siRNA transfected SGC996 cell was 100%. The average tumor volume in experimental group and control group was （277.94±7.6 ） mm3 and （474.47±9.99） mm3 respectively. The growth rate was 7. 941% and 13. 556% ,and the average tumor weight was （0.76 ±0.16） g and （1.78± 0.23） g,respectively. The tumor growth rate,weight and volume in the experimental group decreased significantly when compared with those in control group （P〈0.05）. Conclusion Human gallbladder carcinoma cell line SGC-996 expresses TLR4. SGC-996 cells transfected with siRNA can effectively lower the level of TLR4 gene expression. After silence of TLR4 gene,the growth rate,weight and volume of xenograft are decreased significantly.

关 键 词：TLR4 胆囊癌 RNA干扰 裸鼠 

分 类 号：R735.8[医药卫生—肿瘤]