增殖细胞核抗原蛋白和mRNA在胆管癌中的表达及其意义  

The expression and significance of proliferating cell nuclear antigen in cholangioncarcinom

作　　者：边伟[1] 穆宏凌[2] 刘三光[1] 刘建华[1] 路文彦[1] 

机构地区：[1]河北医科大学第二医院肝胆外科,石家庄050000 [2]河北省卫生厅考试培训中心

出　　处：《中华实验外科杂志》2010年第7期873-875,共3页Chinese Journal of Experimental Surgery

摘　　要：目的 检测胆管癌和癌旁0.5cm胆管组织及手术切缘的正常胆管组织增殖细胞核抗原（PCNA）蛋白及mRNA的表达,探讨其在胆管癌发生发展中的作用.方法 应用免疫组织化学过氧化物酶标记链霉卵白素法（SP）检测42例胆管癌组织及20例正常组织中PCNA蛋白的表达,同时应用逆转录-聚合酶链反应（RT-PCR）技术检测42例术中所取的新鲜的胆管癌、17例同个体癌旁胆管黏膜和20例正常胆管组织中的PCNA mRNA表达,并与临床资料进行相关分析.结果 正常胆管组织、癌旁组织、胆管癌组织中PCNA mRNA相对表达量分别0.5605±0.0331,0.5692±0.0408,0.6303±0.0773,PCNA mRNA在3组之间表达呈上升趋势（P〈0.05）,PCNA蛋白表达趋势同其相对应的基因表达趋势一致,即PCNA mRNA在胆管癌组织中高表达（P〈0.05）.结论 PC-NA基因转录和蛋白可能参与了胆管癌的发生发展过程.Objective To detect the expression of proliferating cell nuclear antigen （PCNA） protein and gene in eholangionearcinoma and to elucidate its role. Methods The express of PCNA protein was detected by Immunohistochemical technique （SP） in 42 cases of eholangionearcinoma and 20 cases normal bile duct,and the express of PCNA gene was detected in 42 cases of eholangionearcinoma, adjacent to eholangionearcinoma and normal bile duct by reverse transcription-polymerase chain reaction （RT-PCR）. The correlation was analysed statistically between the express of PCNA protein and mRNA and clinicopatho-logical parameters in eholangionearcinoma. Results The express of PCNA mRNA in normal bile duct, adjacent to eholangionearcinoma,eholangionearcinoma respectively was 0. 5605 ±0. 0331,0. 5692 ± 0.0408, 0. 6303 ±0.0773,the express of PCNA mRNA was higher in eholangionearcinoma than the others （P〈 0.05）. the protein expression of PCNA was higher in eholangionearcinoma than normal bile duct tissue （P〈0.05）. Conclusion PCNA gene transcription and protein expression is mostly like involved in the proliferation and malignant transformation of bile epithelial and then malignant progression of bile duct carcinomas. The detection of PCNA expression may elucidate the carcinogenesis of bile duct.

关 键 词：胆管癌 PCNA 免疫组织化学 RT-PCR 

分 类 号：R735.8[医药卫生—肿瘤]