四嗪二甲酰胺抑制肺癌细胞株A549、EBC-1、MA增殖并诱导细胞凋亡的研究  

Effects or N, N＇-di-（m-methylphenyi） -3,6-dimethyl-l, 4-dlhydro-l ,2,4,5-tetrazine-l, 4-dicarboam-ide on proliferation and apoptosis of A549, EBC-1, MA cells in vitro

作　　者：许武林[1] 周永列[2] 王珍妮[2] 吕亚萍[3] 胡惟孝[3] 

机构地区：[1]浙江省人民医院呼吸内科,杭州310014 [2]浙江省人民医院中心实验室,杭州310014 [3]浙江工业大学药学院

出　　处：《中华实验外科杂志》2010年第7期936-939,共4页Chinese Journal of Experimental Surgery

基　　金：基金项目：国家自然科学基金资助项目（30973568）;浙江省医药卫生资助项目（2007A008）

摘　　要：目的 观察四嗪二甲酰胺（ZGDHu-1）体外抑制肺癌细胞株A549、EBC-1、MA增殖并诱导细胞凋亡作用.方法 将不同浓度的ZGDHu-1与A549、EBC-1、MA细胞在体外培养,用锥虫蓝染色、噻唑蓝（MTT）比色法、5＇-溴-2脱氧尿苷（HrdU）-酶联免疫吸附试验（ELISA）法观察ZGDHu-1对A549、EBC-1、MA细胞增殖的抑制作用 用细胞形态学、DNA含量及细胞周期分析、Annexin-V/PI双标记、Hoechst33258荧光染色观察细胞凋亡.结果 ZGDHu-1能抑制A549、EBC-1、MA细胞增殖和活力,呈现作用时间和剂量的量效关系.A549、EBC-1、MA细胞经ZGDHu-1作用48 h后,其IC50分别为165、106、210μg/L 72h后的IC50分别为42、18、90μg/L.大部分细胞阻滞于G2～M期 出现典型的细胞形态改变,亚G1峰检出并显著增加,Annexin-V＋/PI-表达升高,细胞内DNA片段含量增加,Hoechst33258荧光染色后出现凋亡细胞的特征性改变,证实ZGDHu-1能诱导A549、EBC-1、MA细胞凋亡.结论 ZGDHu-1能抑制A549、EBC-1、MA细胞增殖,并可诱导其细胞凋亡.Objective To study the effect of N, N-di-（ m-methylphenyi） -3,6-dimethyl-l ,4-di-hydro-1,2,4,5-tetrazine-1,4-dicarboamide （ZGDHu-1） on proliferation and apoptosis of A549, EBC-1 and MA cells in vitro. Methods Different concentrations of ZGDHu-1 were incubated with A549, EBC-1, or MA cells for different durations. The proliferation inhibition was analyzed by alive cell count, Methyl thi-azolyl tetrazolium （MTT） assay and BrdU-ELISA. Cell apoptosis was measured by cell morphology, DNA content, Annexin-V/PI and Hoechst33258 labeling method. Results ZGDHu-1 could inhibit proliferation of A549, EBC-1 and MA cells within a certain range of treating duration and does, with a 48 h IC50 of 165, 106, 210μLg/L, 72 h of 42, 18, 90μg/L, and a majority of A549, EBC-1 and MA cells were arrested in G2/M phase. The apoptosis of A549, EBC-1 and MA cells was confirmed by type cell morphology, DNA fragment, sub-G1 phase and Hoechst33258 and Annexin/PI labeling method. Conclusion ZGDHu-1 can inhibit proliferation and induce apoptosis of A549, EBC-1, and MA cells.

关 键 词：四嗪二甲酰胺 肺癌 增殖 脱噬作用 

分 类 号：R734.2[医药卫生—肿瘤]