Se(IV)对藻蓝蛋白分子的氧化及纳米Se(0)的生成  

Oxidation of phycocyanin by Se(Ⅳ) and formation of nano-Se(0)

作　　者：杨芳[1] 黄峙[2] 郭振江[1] 郑文杰[1] 

机构地区：[1]暨南大学生命科学技术学院化学系,广东广州510632 [2]暨南大学生命科学技术学院生物工程学系,广东广州510632

出　　处：《海洋科学》2010年第6期55-58,65,共5页Marine Sciences

基　　金：国家自然科学基金(20771044);广东省科技计划项目(2007B030703007及2008A030201020);中国博士后基金(20090460787);中央高校基本科研业务费专项资金(21610303)

摘　　要：从钝顶螺旋藻(Spirulina platensis)中提取纯化藻蓝蛋白(Phycocyanin,PC),光吸收值A620/A280为3.1,观察Se(Ⅳ)与PC相互作用的光谱行为及红硒化现象。光谱检测发现,加入SeO32-后,PC在620 nm的特征光吸收减弱,并随Se(Ⅳ)浓度增加和时间延长单调降低,278 nm和347 nm的光吸收增强;PC的荧光发射和荧光激发也均呈现衰减。另外,PC与Na2SeO3相互作用后,观察到红硒化现象,透射电镜(TEM)证明主要存在形态为红色纳米硒。结果提示PC可能是SeO32?作用的敏感靶点,Se(Ⅳ)对PC主要表现为氧化损伤,而Se(Ⅳ)被还原为红色纳米硒。Spectra of interaction between Se（IV） and phycocyanin （PC）, and red Selenium were explored in this paper. PC with an absorbance ratio of 3.1 （A620/A280） was purified from Spirulina platensis. The characteristic absorbance intensity of PC at 620 nm was decreased gradually after SeO3 2- was added, and the intensity decreased with the increases of the concentration and incubation time of Se （IV）. But absorbance at 278 nm and 347 nm was gradually increased. As to fluorescence spectra of PC, both emission peak and excitation peak were decreased. Furthermore, the phenomenon of red selenium transformation was observed, and the dominant form of Se （0） was nano selenium as determined by TEM. These results suggested that PC might be a target of SeO3 2- Se （IV） had oxidative stress to PC and Se （IV） was reduced to red nano selenium.

关 键 词：硒 钝顶螺旋藻 藻蓝蛋白 光谱 纳米 

分 类 号：Q51[生物学—生物化学]