31例乳腺癌CD44v6和CD44intron 9检测  

Detection of CD 44v6 and CD44 intron 9 in 31 Cases of Breast Cancer

作　　者：周立娜[1] 赵晶[2] 赵维诚[2] 周强 

机构地区：[1]中国医科大学生物技术研究所,沈阳110001 [2]辽宁省肿瘤医院,沈阳110042 [3]辽宁省凌海市中医院,凌海121200

出　　处：《浙江肿瘤》1999年第1期41-41,共1页

摘　　要：［目的］探讨 ＣＤ ４４ ｍＲＮＡ异常表达和拼接与乳腺癌的相关意义。［方法］采用 ｒｅｖｅｒｓｅ ｔｒａｎｓｃｒｉｐｔｉｏｎ－ ｐｏｌｙｍｅｒａｓｅ ｃｈａｉｎｒｅａｃｔｉｏｎ（ＲＴ－ＰＣＲ）方法检测了３１例乳腺癌组织、１７例乳腺癌周组织中ＣＤ ４４ｖ６和ＣＤ ４４ ｉｎｔｒｏｎ ９的表达。［结果］乳腺癌患者癌组织、癌周组织中ＣＤ４４ｖ６的表达率均为１００％（３１／３１，１７／１７），ＣＤ４４ ｉｎｔｒｏｎ９的表达率分别为８３．８７％（２６／３１），９４．１２％（１６／１７），经ｘ２检验，ＣＤ ４４ｖ６、 ＣＤ ４４ ｉｎｔｒｏｎ ９在乳腺癌及癌周组织中的表达差异无显著性。［结论］ ＣＤ ４４ｖ６、 ＣＤ ４４ ｉｎｔｒｏｎ ９在乳腺癌患者中的表达具有普遍性，其表达与乳腺癌的转移无显著相关性。Purpose] To study the correlation between the CD 44 gene abnormal expression, splicing and the breast cancer. [Method] We examined CD 44 gene expressions in samples from breast carcinomas in 31 cases and from its corresponding peri - caroinoma tissues in 17 cases using reverse transcription followed by polymerase chain reaction (PCR). [Results] The results showed that CD 44v6 mRNA was expressed in 31 of 31 (100% ) breast cancers and in 17 of 17 (100% ) corresponding peri - carcinoma tissues the intron 9 in CD 44 mRNA transcripts was detected in 26 of 31 (83. 87% ) carcinomas and in 16 of 17 (94. 12% ) corresponding peri - carcinoma specimens. [Conclusion] The results suggest that the expressions of CD 44v6 and CD44 intron 9 are very common in breast cancers. No significant relationship between breast carcinoma metastasis and the expression of CD 44v is found.

关 键 词：CD44 变异性剪接 乳腺肿瘤 肿瘤转移 

分 类 号：R737.9[医药卫生—肿瘤]