雌激素对实验性骨质疏松骨组织Ⅰ型胶原和基质金属蛋白酶-9表达的影响  

Effects of estrogen on the expression of type 1 collagen and matrix metalloproteinase 9 in bone tissues of experimental osteoporosis

作　　者：赵海波[1,2] 夏志道[1,2] 蔡国平[1,2] 汪岚[1,2] 杜靖远 朱通伯[1,2] 

机构地区：[1]同济医科大学附属协和医院骨科 [2]清华大学生物科学与技术系细胞生物学室

出　　处：《中华老年医学杂志》1999年第1期42-42,共1页Chinese Journal of Geriatrics

基　　金：卫生部科研基金

摘　　要：目的探讨绝经后骨质疏松症发生的分子机制以及雌激素作用的分子环节。方法通过斑点杂交和原位杂交技术，研究卵巢摘除大鼠骨组织Ⅰ型胶原和基质金属蛋白酶－９（ＭＭＰ－９）ｍＲＮＡ表达及分布。结果骨质疏松时骨组织Ⅰ型胶原较正常组增加３倍，而ＭＭＰ－９表达均较正常组高４倍，雌激素替代治疗后，两基因表达下降。Ⅰ型胶原ｍＲＮＡ定位于成骨细胞，而ＭＭＰ－９则主要在破骨细胞、衬细胞和一些骨髓单核细胞（包括破骨细胞前体细胞）中表达。结论雌激素可通过影响骨形成和骨吸收相关基因的表达水平，降低骨转换率。Objective To elucidate the molecular mechanism of postmenopausal osteoporosis and the molecular links of estrogen replacement in vivo. Methods An adult ovariectomied rat model was used in this study. The expression and distribution of type 1 collagen and matrix metalloproteinase 9(MMP 9) mRNA in bone tissues were analysed by dot blot and in situ hybridization respectively. Results The expression levels of both type 1 collagen and MMP 9 were higher in osteoporotic bone tissues than in normal control group. While treated with estrogen, the expression of both genes declined to some degree. Type 1 collagen mRNA located in osteoblasts, whereas MMP 9 was mainly expressed in osteoclasts, some lining cells on bone surface and some mononuclear cells in bone marrow, including mononuclear osteoclastic precursor cells. Conclusions Estrogen plays its role in prevention and treatment of osteoporosis by reducing bone turnover rate while affecting the expression levels of genes related to bone formation and resorption.

关 键 词：骨质疏松 绝经后 雌激素 基因表达调控 酶学 

分 类 号：R681.02[医药卫生—骨科学]