骨髓间充质干细胞对大鼠胰腺损伤的修复  

Bone marrow mesenchymal stem cells in repair of injured pancreas in rats

作　　者：陈强[1] 王秋静[2] 杨东旭[3] 周余来[4] 

机构地区：[1]吉林大学公共卫生学院毒理学教研室,吉林省长春市130021 [2]吉林大学白求恩医学院,吉林省长春市130021 [3]吉林大学公共卫生学院 [4]吉林大学药学院,吉林省长春市130021

出　　处：《中国组织工程研究与临床康复》2010年第23期4252-4256,共5页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：国家"863计划"资助项目(2004AA205020)~~

摘　　要：背景:胰腺缺血性损伤是临床常见但又难以解决的实际问题,常规的预防和治疗方法收效甚微。骨髓间充质干细胞是近年来兴起的治疗损伤公认有效的手段,因此,有必要寻找利用干细胞治疗缺血性胰腺损伤的方法。目的:观察骨髓间充质干细胞对胰腺缺血损伤的修复作用。方法:采用贴壁培养和组织块培养相结合的方法得到高纯度的骨髓间充质干细胞,在含体积分数为20%胎牛血清的L-DMEM培养液中扩增培养,传3代后用增强型绿色荧光蛋白重组腺病毒标记细胞,注入胰腺缺血性损伤动物模型胰腺局部,观察动物存活率,冰冻切片共聚焦显微镜观察标记细胞位置。结果与结论:实验分离获得了高纯度贴壁生长的骨髓间充质干细胞。注入细胞的动物模型存活率明显高于对照组;共聚焦显微镜观察显示,注入细胞24h后,细胞可到达受损部位,并聚集在微循环系统中,以后逐渐大量进入受损部位,在循环系统及导管系统中有较高表达。提示采用骨髓间充质干细胞局部注入,可明显增加模型动物的存活率,有利于损伤的胰腺修复。BACKGROUND：The ischemic injury of pancreas is a common practical problem in the clinic,which is difficult to solve.The routine methods of prevention and treatment get little success.Bone marrow mesenchymal stem cells （BMSCs） are a widely accepted modus that is developed in the recent years to treat injury,so it is necessary to find an effective way to treat the ischemic injury of pancreas using stem cells.OBJECTIVE：To study the repair effect of BMSCs on ischemic injury of pancreas.METHODS：Adherent culture and tissue culture were used to get highly purified BMSCs,which were incubated in L-DMEM containing 20% fetal bovine serum.Following three subcultures,BMSCs were labeled by enhanced green fluorescent protein recombinant adenovirus by injection into local pancreas of animal models where ischemic injury happened.Survival rate of the rats was observed,and the position of the marked cells in frozen sections was observed by confocal microscopy.RESULTS AND CONCLUSION：Highly purified BMSCs were harvested from adherent culture.The survival rate was significantly higher in injected animals than the control rats.The results of confocal microscopy indicated that after 24 hours of the injection,the cells reached the ischemic part and aggregated in the microcirculatory system,and then entered the injured part gradually and heavily,finally got highly expressed in the microcirculatory system and conduit system.Results have suggested that the survival rate of the rats can be heightened by local injection of BMSCs,and this is propitious to the recovery of ischemic pancreas.

关 键 词：胰腺 骨髓间充质干细胞 缺血 修复 大鼠 

分 类 号：R394.2[医药卫生—医学遗传学]