人脐血单个核细胞移植前后患者脑脊液中细胞生长因子的变化  

Changes in cellular growth factor levels in cerebrospinal fluid of the patients before and after human umbilical cord blood mononuclear cell transplantation

作　　者：李强[1] 吕涌涛 郇英[1] 曹毓林 刘敏[1] 孙良伟[1] 陈兴旺[1] 胡祥 葛汝村[1] 

机构地区：[1]山东省交通医院神经内科,山东省济南市250031 [2]深圳北科生物细胞工程研究所,广东省深圳市518057

出　　处：《中国组织工程研究与临床康复》2010年第23期4267-4270,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：背景:移植的神经干细胞可以通过分泌或调节某种或几种神经营养因子来促进损伤后神经功能的修复。目的:检测人脐血单个核细胞治疗患者脑脊液中神经生长因子、血管内皮生长因子、碱性成纤维细胞生长因子的水平。方法:选择山东省交通医院行人脐血单个核细胞治疗的神经系统疾病患者28例,于腰穿蛛网膜下腔途径行脐血单个核细胞治疗1次/周,共治疗3次,每次治疗前留取脑脊液2mL。采用酶联免疫吸附法(ELlSA)检测人脐血单个核细胞治疗患者脑脊液中神经生长因子、血管内皮生长因子、碱性成纤维细胞生长因子水平的变化,并观察移植后不良反应。结果与结论:经单因素方差分析及组间q检验,第2次治疗1周后,神经生长因子水平明显高于治疗前及第1次治疗后1周(P<0.05);第2次治疗1周后,血管内皮生长因子和碱性成纤维细胞生长因子水平均高于治疗前及第1次治疗后1周,但差异无显著性意义(P>0.05)。脐血单个核细胞移植后出现低热12例,头痛4例,腰痛3例,乏力4例,恶心、呕吐2例,均为一过性症状,数日内自行缓解。结果显示,人脐血单个核细胞治疗患者脑脊液中神经生长因子水平有明显增高,血管内皮生长因子及碱性成纤维细胞生长因子的含量变化不明显。BACKGROUND：Transplanted neural stem cells can contribute to repair of neural function following damage through secreting or regulating some or several neurotrophic factors.OBJECTIVE：To detect levels of nerve growth factor （NGF）,vascular endothelial growth factor （VEGF） and basic fibroblast growth factor （bFGF） in cerebrospinal fluid in patients treated with umbilical cord blood mononuclear cells.METHODS：A total of 28 patients with nervous system disease treated with human umbilical cord blood mononuclear cells at the Shandong Traffic Hospital were enrolled.They received treatment of human umbilical cord blood mononuclear cells via lumbar puncture subarachnoid cavity,once a week,for three times.A total of 2 mL cerebrospinal fluid was maintained before each treatment.Changes in NGF,VEGF and bFGF levels were determined in patients treated with human umbilical cord blood mononuclear cells using enzyme linked immunosorbent assay,and adverse reactions were observed following transplantation.RESULTS AND CONCLUSION：NGF levels were significantly greater at 1 week after the second treatment than pretreatment and 1 week after the first treatment using one-way analysis of variance and intergroup q-test （P0.05）.VEGF and bFGF levels were higher at 1 week after the second treatment than pretreatment and at 1 week after the first treatment （P0.05）.There were 12 cases of low-grade fever,4 cases of headache,3 cases of lumbago,4 cases of debility and 2 cases of nausea and vomiting.All were transitory symptoms,which were self relieved within several days.Results have displayed that NGF levels in cerebrospinal fluid were significantly increased in patients treated with umbilical cord blood mononuclear cells,however,VEGF and bFGF levels were not influenced significantly.

关 键 词：神经生长因子 脐血干细胞 血管内皮生长因子 脑脊液 细胞移植 碱性成纤维细胞生长因子 

分 类 号：R394.2[医药卫生—医学遗传学]