蛋白酶体抑制剂MG-132作用下失神经骨骼肌myf-5的表达  

Effect of proteasome inhibitor MG-132 on myf-5 protein expression in rat denervated skeletal muscles

作　　者：阎少君[1] 王东[1] 张文辉[1] 

机构地区：[1]山西医科大学第三医院骨科,山西省太原市030053

出　　处：《中国组织工程研究与临床康复》2010年第24期4469-4472,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：背景:MG-132是蛋白酶体抑制剂的一种,有报道显示其对失神经骨骼肌萎缩有延缓作用。目的:进一步验证蛋白酶体抑制剂MG-132对大鼠骨骼肌成肌调节因子myf-5基因表达的影响。方法:SD大鼠随机被分成3组,空白对照组不切断坐骨神经,仅做假手术,去神经组和去神经MG-132干预组切断坐骨神经1cm以上,制作失神经骨骼肌动物模型,去神经MG-132干预组肌肉内注射MG-132。分别于去神经第2,7,28d处死大鼠。用反转录聚合酶链式反应技术检测myf-5mRNA表达情况,Western-blot检测myf-5蛋白表达的变化。结果及结论:在去神经支配后第2,7,28天,与空白对照组比较,去神经组、去神经MG-132干预组myf-5mRNA和蛋白质均表达上调(P<0.01);去神经MG-132干预组myf-5mRNA和蛋白表达较去神经组均明显上调(P<0.01)。结果提示蛋白酶体抑制剂MG-132可以通过抑制泛素-蛋白酶体途径来上调myf-5的表达,从而起到延缓骨骼肌萎缩的作用。BACKGROUND：MG-132 is a kind of proteasome inhibitor,which has delaying effect on atrophy of denervated skeletal muscle.OBJECTIVE：To detect the effect of proteasome inhibitor MG-132 on expression of myogenic regulatory factors Myf-5 gene of denervated skeletal muscle in rats.METHODS：Sprague-Dawley rats were randomly divided into three groups.Sham operation were made only for the rats in the control group（sciatic nerves were not cut off）.Sciatic nerves were cut off more than 1-cm at the mid-level on their right lower limbs for the rats in the denervated and MG-132 intervention groups.Rats were sacrificed at 2,7,and 28 days after operation.The expression of Myf-5 mRNA was detected by RT-PCR,and the changes of myf-5 protein were determined by Western blot.RESULTS AND CONCLUSION：Compared with the control group,the expressions of Myf-5 mRNA and protein were increased in the denervated and MG-132 intervention groups at 2,7,and 28 days after operation（P 0.01）.The increasing of Myf-5 mRNA in the MG-132 intervention group was greater than that in the denervated group（P 0.01）.The results suggested that the proteasome inhibitor MG-132 can inhibit the ubiquitin-proteasome pathway to increase the expression of Myf-5 gene in order to play the role of slowing skeletal muscle atrophy.

关 键 词：去神经支配 myf-5 肌萎缩 蛋白酶体抑制剂 肌肉肌腱组织工程 

分 类 号：R318[医药卫生—生物医学工程]