应用改良质子磁共振波谱技术研究帕金森病大鼠纹状体内神经代谢的变化  

Biochemical Changes in Striatum of Parkinson's Disease Rat Model Observed by Modified Proton Magnetic Resonance Spectroscopy

作　　者：郑志娟[1,2] 付文玉[1] 王峻清[3] 孙西河[4] 庄文欣[1] 吕娥[1] 杨丽[1] 吕翠[1] 

机构地区：[1]潍坊医学院山东省"泰山学者"设岗学科组织胚胎学教研室,潍坊261053 [2]山东中医药大学教学实验中心 [3]山东中医药大学基础医学院,济南250355 [4]潍坊医学院附属医院影像中心,潍坊261031

出　　处：《生物医学工程学杂志》2010年第3期524-528,共5页Journal of Biomedical Engineering

基　　金：山东省自然科学基金资助项目(Y2003C01,Y2008C129);山东省“泰山学者”建设工程专项经费资助;潍坊医学院博士启动基金资助

摘　　要：利用改良的氢质子磁共振波谱技术观察帕金森病大鼠纹状体内神经代谢的变化。6-羟多巴胺单侧黑质注射制备的帕金森病大鼠模型7只作为实验组,5只正常大鼠设为对照组。采用改良的1.5Tesla双梯度超导型磁共振成像仪对两组大鼠双侧纹状体行氢质子磁共振波谱检测,免疫组织化学技术检测黑质及纹状体酪氨酸羟化酶变化。结果显示,实验组大鼠损毁侧黑质致密部酪氨酸羟化酶阳性神经元及纹状体酪氨酸羟化酶阳性纤维明显减少(P<0.05),证明模型制作成功。损毁侧纹状体内N-乙酰天门冬氨酸/肌酸比值和N-乙酰天门冬氨酸浓度明显低于健侧(P<0.05),双侧纹状体内胆碱类化合物/肌酸比值无显著差别。结果表明,氢质子磁共振波谱可以进行活体连续化无创检测,改良的1.5Tesla磁共振波谱技术可以为偏侧帕金森病大鼠模型纹状体细胞物质代谢改变提供有价值的信息。This study was undertaken to observe the biochemical changes in striatum of Parkinson＇s disease（PD） rat model by modified proton magnetic resonance spectroscopy. 12 SD rats were divided into model （n=7） and control （n=5） groups. At 3 weeks after the injection of 6-hydroxydopamine into right striatum,1H-MRS on the striatum was taken by modified proton magnetic resonance spectroscopy,and then tyrosine hydroxylase（TH） immunostatining was used to visualize the changes of the neurons in substantia nigra and neurites in striatum. The results showed that TH positive neurons and neurites in the substantia nigra compacta （SNc） and striatum in the normal side of the rat model of PD were decreased （P0.05）,which proved the successful establishment of PD models. The NAA/Cr ratio of the injected side striatum of model group was lower than that of the normal side （P0.05）. The ratios of Cho/Cr showed no significant difference between the two sides （P0.05）. These results indicated that the modified 1.5T 1H-MRS should be a noninvasive technique which could provide useful information about the biochemical metabolites in striatum for the study of PD in rat model.

关 键 词：帕金森病 氢质子磁共振波谱 酪氨酸羟化酶 免疫组织化学 大鼠 

分 类 号：R742.5[医药卫生—神经病学与精神病学]