心理应激复合睡眠剥夺对大鼠学习记忆能力和部分营养素的影响  

Effects of psychological stress combined with sleep deprivation on learning memory ability and certain nutrients in rats

作　　者：胡家庆[1] 徐灵活[1] 王晓花[1] 欧益伊[2] 周宏元[1] 章建程[1] 殷明[3] 

机构地区：[1]海军医学研究所,上海200433 [2]第二军医大学军队卫生教研室 [3]海后卫生部

出　　处：《中华航海医学与高气压医学杂志》2010年第3期140-142,共3页Chinese Journal of Nautical Medicine and Hyperbaric Medicine

基　　金：基金项目：全军医药卫生“十一五”科研基金课题（06MA012）

摘　　要：目的 探讨连续14 d心理应激复合48 h睡眠剥夺对大鼠学习记忆和部分营养素的影响.方法 45只雄性SD大鼠随机分为空白对照组、复合因素组和复合阴性组,每组15只.采用Communication Box和改良后的小平台法建立心理应激复合睡眠剥夺模型.利用Morris水迷宫进行训练以检测大鼠的学习记忆能力.取大鼠血清测定Vc、Ve、Fe含量及CK活力.结果 复合因素干预后,大鼠4个象限总逃逸潜伏期、第1象限逃逸潜伏期及第2象限逃逸潜伏期明显延长,Fe含量降低.结论 14 d心理应激复合48 h睡眠剥夺干预损害大鼠学习记忆功能,导致部分营养素缺乏.Objective To investigate effects of successive 14 d psychological stress combined with 48 h sleep deprivation on learning memory and certain nutrients in rats.Methods Forty-five male SD rats were randomly divided into 3 groups,each consisting of 15 animals;the blank control group,the multi-factor group and the multi-factor negative control group.The psychological stress coupled with sleep deprivation model was developed with the Communication Box and a small modified platform.Learning memory of rats was measured by the Morris water maze.Serum samples were taken from the rats to measure levels of Vc、Ve、Fe and CK activity.Results Following multi-factor intervention,escape latency of rats for total-quadrant,the first and the second quadrant were significantly longer than those of the control group,and Fe concentration decreased obviously.Conclusions Fourteen-day psychological stress coupled with 48-hour sleep deprivation could impair learning memory of rats,and induced deficiency of certain nutrients in rats.

关 键 词：心理应激 睡眠剥夺 学习记忆能力 营养素 

分 类 号：R686[医药卫生—骨科学]