大鼠急性心肌缺血/再灌注模型改良方法与传统方法的比较  

Comparison between conventional and modified methods of acute myocardial ischemia/reperfusion models

作　　者：刘海涛[1] 李飞[1] 王跃民[2] 陶凌[1] 李聪叶[1] 苑媛[1] 王海昌[1] 

机构地区：[1]第四军医大学西京医院心血管内科,陕西西安710032 [2]第四军医大学基础部生理教研室,陕西西安710032

出　　处：《心脏杂志》2010年第4期533-536,共4页Chinese Heart Journal

基　　金：国家自然科学基金项目课题(30770784)

摘　　要：目的:建立大鼠急性心肌缺血/再灌注模型的改良方法与传统方法并进行比较。方法:将60只SD大鼠随机分为两组,每组30只;一组采用改良方法建立模型,即气体麻醉,以气体麻醉面罩通气,短时间开胸;另一组采用传统方法建立模型,即腹腔麻醉,气管插管,长时间开胸。比较两种方法的手术时间、手术成功率、术中血氧饱和度、术后存活率、心肌梗死面积,以及术后大鼠的生活状况。结果:与传统方法组相比,改良方法组大鼠术中存活率升高[(93.3±4.6)%vs.(72.4±8.4)%,P<0.05]、开胸时间缩短为[(6.5±2.0)minvs.(44.9±2.7)min,P<0.01],开胸前后平均血氧饱和度增加[(96.3±0.8)%vs.(90.9±2.1)%,P<0.05],术后存活率提高(82.83%vs.58.3%,P<0.01)。另外改良方法组大鼠术后恢复正常行为活动需要的时间较短,但两组大鼠心肌梗死的面积[(33.5±2.2)%vs.(35.0±3.0)%]无统计学意义。结论:改良方法具有简单、高效及可独立操作的优点,能有效提高建立大鼠急性心肌缺血/再灌注模型的成功率。AIM： To develop and compare the efficacy of a modified vs. conventional rat model of acute myocardial ischemia/reperfusion. METHODS： Sixty Sprague Dawley （SD） rats were randomly divided into two groups： a modified group （respiratory mask support and short-time chest opening） and a conventional group （tracheal intubation and long-time chest opening）. Surgical time, surgical success rate, survival rate, and infarct size were investigated, and the postoperative survival rate of the rats was observed. RESULTS： During the perioperative period, the surgical success rate was higher in the modified model [（93.3±4.6）% vs. （72.4±8.4）%, P〈0.05]. Chest-opening time was shorter in the modified group vs. the conventional model [（6.5±2.0） min vs. （44.9±2.7） min, P〈0.01]. Postoperative resumption of normal behaviors and activities was quicker in the modified group, with a statistically significant higher survival rate compared with that in the conventional group （82.83% vs. 58.3%, P〈0.001）. Triphenyltetrazolium chloride staining showed no significant difference in infarct size between groups [（33.5±2.2）% vs. （35.0±3.0）%]. CONCLUSION： The modified method offers the advantages of simplicity, efficiency and independent operation, which enhances the success rate of establishing the acute rat myocardial ischemia/reperfusion model.

关 键 词：心肌缺血/再灌注 模型 改良方法 传统方法 大鼠 

分 类 号：R542.2[医药卫生—心血管疾病]