丙泊酚对胎儿型和α7烟碱型乙酰胆碱受体的作用  

Effects of Propofol on Embryonic-and α7-Nicotinic Acetylcholine Receptors

作　　者：赵雪莲[1] 庄心良[2] 安广权[3] 高雁[1] 徐国辉[2] 邢玉英[1] 

机构地区：[1]河北医科大学第四医院麻醉科,050011 [2]上海交通大学第一附属医院麻醉科 [3]河北医科大学第四医院外二科

出　　处：《天津医药》2010年第7期602-604,共3页Tianjin Medical Journal

基　　金：河北省重点特色学科建设项目(项目编号:[2005]52);河北省卫生厅指导性项目(项目编号:20090533)

摘　　要：目的:通过重组受体,观察丙泊酚对胎儿型乙酰胆碱受体和神经型α7乙酰胆碱受体的作用。方法:通过脂质体转染法在HEK293细胞表达胎儿型或α7乙酰胆碱受体。应用全细胞电压-膜片钳技术研究不同浓度(0.1μmol/L～3mmol/L)丙泊酚对2种受体亚型的影响。结果:临床相关浓度丙泊酚对100μmol/L乙酰胆碱激发胎儿型和α7乙酰胆碱受体的电流均有抑制作用。不同浓度丙泊酚对胎儿型或α7乙酰胆碱受体电流抑制作用比较差异无统计学意义(P>0.05)。相同浓度丙泊酚对胎儿型乙酰胆碱受体抑制大于对α7乙酰胆碱受体的抑制(P<0.05)。结论:丙泊酚对γ-nAChR和a7-nAChR都有抑制作用,抑制程度与丙泊酚的浓度无关。Objective：To investigate the effect of propofol on embryonic-and α7-nicotinic acetylcholine receptors with whole cell clamp patch.Methods：The embryonic-and α7-nicotinic acetylcholine receptors were heterologously expressed in HEK293 cells and activated to maximal currents by the application of acetylcholine（100 μmol/L）.Peak currents were recorded in HEK293 cells treated by propofol using whole cell patch clamp technique.Results：Propofol produced rapid and readily reversible,concentration-dependent inhibition.The inhibitory effect of propofol was greater to embryonic-nicotinic acetylcholine receptor compared to that of α7-nicotinic acetylcholine receptor（P 〈0.05）.Propofol wasn＇t resulted in a concentration-dependent inhibition in embryonic-and α7-nicotinic acetylcholine receptors（P〉 0.05）.Conclusion：There was inhibitory effect of propofol on embryonic-and α7-nicolinic acetycholine receptors,which was independent of the concentration of propofol.

关 键 词：二异丙酚 受体 胆碱能 受体 烟碱 脂质体 细胞系 转染 膜片钳术 

分 类 号：R749.16[医药卫生—神经病学与精神病学]