4周有氧运动干预对大鼠非酒精性脂肪肝和肝内细胞色素P450 2E1的影响  

Effects of 4-week aerobic exercises intervention on rats non-alcoholic fatty liver disease and hepatic cytochrome P450 2E1

作　　者：史艳莉[1] 余辉[2] 张红菱[1] 

机构地区：[1]武汉工业学院健康科学与护理系,湖北武汉430026 [2]黄石市第一医院内科

出　　处：《中国康复医学杂志》2010年第7期646-649,共4页Chinese Journal of Rehabilitation Medicine

摘　　要：目的:探讨4周有氧运动干预对大鼠非酒精性脂肪肝(NAFLD)和肝内细胞色素P4502E1(CYP2E1)的影响。方法:实验大鼠随机分为对照组、模型组和模型+运动组。模型组和模型+运动组经6周脂肪乳灌胃,制成非酒精性脂肪肝模型。随后模型+运动组进行为期4周有氧运动干预。检测各组大鼠胆固醇、甘油三酯、肝脏组织病理改变和肝组织CYP2E1表达。结果:造模组大鼠体重及血脂明显增高(P<0.05),肝脏病理学检查证实非酒精性脂肪肝模型造模成功。模型组及模型+运动组肝脏CYP2E1明显较对照组增高(P<0.05)。经过4周游泳训练后,模型+运动组大鼠与模型组比较胆固醇、甘油三酯和肝脏CYP2E1呈下降趋势(P<0.05);肝脏病理学检查提示脂肪肝较模型组明显好转。结论:有氧运动不仅能够改善高脂血症状态,也能降低CYP2E1表达,可以预防和治疗NAFLD时肝组织所受的过氧化损伤。Objective：To investigate the effects of aerobic exercises on liver tissue and cytochrome P-450 2E1（CYP2E1） in rats with non-alcoholic fatty liver disease（NAFLD） induced by high fat diet.Method：The rats were randomly divided into normal group,model group and model ＋ exercises group.Aerobic exercises intervention was applied on the model＋exercises group for 4 weeks.Total cholesterol（TC）,triglyceride（TG）,liver pathological changes and liver tissue CYP2E1 expression were detected in each group.Result：After six weeks lipid emulsion gavage,the level of body weight,TC and TG rised evidently（P0.05）,the liver pathological changes indicated NAFLD model was produced successfully,the level of hepatic cytochrome P450 2E1 rised evidently compared with control group（P0.05）.After four-week aerobic exercises,the level of TC,TG and cytochrome P450 2E1 declined evidently（P0.05） and the liver pathological changes improved significantly.Conclusion：Aerobic exercises not only can improve hyperlipidemia,but also can reduce CYP2E1 expression,prevent and treat peroxidative injury of liver tissue in NAFLD.

关 键 词：有氧运动 非酒精性脂肪肝 高脂血症 细胞色素 

分 类 号：R575.5[医药卫生—消化系统] R493[医药卫生—内科学]