黑木耳多糖AAPS-3的化学结构  

Chemical structure of polysaccharide AAPS-3 from Auricularia auricula-judae

作　　者：宋广磊 杜琪珍 

机构地区：[1]浙江工商大学食品与生物工程研究所,杭州310035

出　　处：《菌物学报》2010年第4期576-581,共6页Mycosystema

基　　金：国家"863计划"专题(No2007AA10Z342)

摘　　要：利用逆流色谱分离技术从黑木耳中分离了黑木耳多糖AAPS-3。通过完全水解、甲基化、一维和二维1H和13C核磁共振(NMR)阐明AAPS-3的化学结构。经完全水解证实AAPS-3主要由Glc单糖组成,用HPLC方法测得其分子量为4.83×105,通过甲基化反应数据和1H和13C NMR等分析发现AAPS-3为由β-D-1,4-Glc,β-D-1,3-Glc和β-D-1,6-Glc残基构成主链的葡聚糖。A homogeneous polysaccharide AAPS-3 was obtained from Auricularia auricula-judae by countercurrent chromatography technology.The structure of AAPS-3 was elucidated using the method of complete acid hydrolysis,methylation,and 1D and 2D nuclear magnetic resonance（NMR）.AAPS-3 was found to be mainly composed of Glc by means of total acid hydrolysis.Its molecular weight was estimated to be 4.83×105 by HPLC method.Methylation analysis and 1H and 13C NMR indicated the main chain of AAPS-3 consisted of β-D-1,4-Glc,β-D-1,3-Glc and β-D-1,6-Glc.

关 键 词：食药用菌 逆流色谱 核磁共振 

分 类 号：S646.6[农业科学—蔬菜学]