柔毛霉素对兔晶体上皮细胞体外生长抑制的实验研究  

A study of dounomycin on inhibition of rabbit lens epithelial cell proliferation in culture

作　　者：马进 陈大本 马华 

机构地区：[1]蚌阜医学院附属医院眼科

出　　处：《中国实用眼科杂志》1999年第1期59-60,共2页Chinese Journal of Practical Ophthalmology

摘　　要：目的：研究柔毛霉素（ｄａｕｎｏｍｙｃｉｎ）对兔晶体上皮细胞体外生长抑制，探索使用柔毛霉素以预防后发障的发生。方法：分离培养家兔晶体上皮细胞，传代后在２４孔培养板培养７２小时，计数后加入不同浓度的柔毛霉素作用１０分钟后，吸出药物并冲洗后培养５天，结果：柔毛霉素的ＬＤ５０为０．５μｇ／ｍｌ～０．７７μｇ／ｍｌ，经高浓度药物（１０μｇ／ｍｌ）作用后的标本几乎无细胞存活；经０．５μｇ／ｍｌ药物浓度作用后的细胞形态发生变化。结论：显示出低浓度柔毛霉素短时间作用后即能有效抑制晶体上皮细胞的增生。Objective:To study the ability of daunomycin on inhibiting the proliferation of rabbit lens epithelial cells in culture and prevention of after cataract by using daunomycin.Methods:rabbit lens epithelial cells were isolated and cultured and the passege cells were placed in 24-well tissue culture plates and incabated for 72 hours,counted and exposed to different concentration of daunomycin for 10 minutes.After the drug containing medium was aspirated and each well was washed,the cell cultured 5 days.Results:The calculated LD50 for the drug against rabbit lens epithelial cell wad 0 52 μg/ml～0 77 μg/ml.Very few viable cell remained after exposure to higher concentrations of the drug(10 μg/ml)Conclusion:Daunomycin,at low concentration has been shown to be an effective inhibitor of rabbit lens epithelial cells proliferation in culture useing an exposure time of ten minutes.The authors consider that daunomycin may be effective for the prevention of after cataract and in especial a single intraoperative infusion of the drug surgery might be sufficient.

关 键 词：柔毛霉素 晶体上皮细胞 兔 白内障 

分 类 号：R776.106[医药卫生—眼科]