胃癌CD_(44)v6表达及其临床意义的研究  

Expression of CD44 Splice Varian v6 in Human Gastric Cancer

作　　者：张成武[1] 徐文娟[1] 赵仲生[1] 邹寿椿[1] 

机构地区：[1]浙江省人民医院外科

出　　处：《中国肿瘤临床》1999年第2期91-94,共4页Chinese Journal of Clinical Oncology

摘　　要：目的：探讨胃癌ＣＤ４４ｖ６的表达，评价其表达对胃癌进展程度和转移的诊断意义，以及对手术预后的影响。方法：收集胃癌手术标本８５例，相应的癌转移淋巴结标本４７例，应用Ｓ－Ｐ免疫组化染色技术检测ＣＤ４４ｖ６蛋白在上述组织中的表达。结果：胃癌原发灶ＣＤ４４ｖ６阳性表达率为７４．１％（６３／８５），淋巴结转移灶阳性表达率为８３．０％（３９／４７），Ｌａｕｒｅｎ分型肠型胃癌和弥漫型胃癌的阳性率分别为８０．０％（４４／５５）和４７．６％（１０／２１），肠型胃癌阳性率显著高于弥漫型（Ｐ＜０．０５），ＣＤ４４ｖ６阳性表达与淋巴结阳性转移率有关，其染色强度与ＴＮＭ分期相关，ＣＤ４４ｖ６阳性者术后３年生存率低于阴性者（Ｐ＜０．０５）。结论：对胃癌组织进行ＣＤ４４ｖ６蛋白的检测，有助于预测胃癌进展程度和淋巴结转移的诊断，以及评估胃癌患者的预后。Objective: To evaluate the expression of CD44 splice variant v6 in gastric cancer and its role in the estimation of tumor progression, metastasis as well as prognosis. Methods: The expression of CD44v6 protein was determined immunohistochemically in 85 surgically resected primary tumor specimens and 47 excised metastatic lymph nodes of gastric cancer using murine exon v6-specific monoclonal antibody. Results: The positive rate of CD44v6 immunoreactivity was 74.1%(63/85) and 83.0%(39/47)in primary gastric cancer and metastatic lymph nodes respectively. The positive rate of CD44v6 immunoreactivity was 80.0%(44/55) and 47.6%(10/21) in the intestinal-type and diffuse-type tumors respectively as classified by Lauren.The positive rate of CD44v6 immunoreactivity in the intestinal-type cancer was higher than that of the diffuse-type significantly (P< 0.05). The presence of CD44v6 protein was correlated with the lymph node involvement ratio (ie the ratio between the number of excised and involved lymphnodes). The staining intensity of CD44v6 was relatedwith the UICC TNM classification. Patients with CD44v6 positive tumor had low 3-year survival rate (P< 0.05). Conclusion: Determination of CD44v6 protein by immunohistochemistry might be useful in assessing tumor progression and lymphatic metastasis and in predicting prognosis of gastric cancer. In addition, the results of our study support the hypothesis that diffuse-type and intestinal-type carcinoma of the stomach arise from different precursor cells of the normal stomach mucosa and progress by different pathways.

关 键 词：胃肿瘤 CD44蛋白 免疫组织化学 

分 类 号：R735.202[医药卫生—肿瘤]