家禽血液基因组DNA的快速提取  

Rapid Extraction of Genome DNA from Avian Blood

作　　者：孙朕[1] 孙英民[1] 郑素月[1] 樊宝良[1,2] 

机构地区：[1]河北农业大学动物科技学院,河北保定071001 [2]河北工程大学农学院,河北邯郸056038

出　　处：《安徽农业科学》2010年第16期8449-8452,共4页Journal of Anhui Agricultural Sciences

基　　金：国家"863"计划"家禽和昆虫蛹生物反应器"项目(2007AA100504);河北省自然科学基金项目(2008000713);河北科学技术研究与发展计划(07220401D-9)资助

摘　　要：[目的]建立一种应用硅胶膜提取禽血DNA的新方法。[方法]应用硅胶膜法和传统的酚/氯仿法提取抗凝禽血基因组DNA,设计合成了一对2.7 kb的鸡卵清蛋白基因的引物,检测这2种方法提取的基因组DNA模板对聚合酶链式反应(PCR)扩增效率的影响。[结果]用硅胶膜法提取的基因组DNA产量和质量均比传统的酚/氯仿法高,将该法提取的基因组DNA作为模板能够消除抗凝剂对后续PCR反应的影响。[结论]硅胶膜法为快速提取高质量的禽血基因组DNA奠定了基础。[Objective] To establish a novel method for extracting genome DNA from avian blood sample by silica membrane.[Method] The extraction of genome DNA from EDTA and Heparin anticoagulated chicken blood were performed by the silica membrane adsorption method and phenol-chloroform extraction method.A pair of 2.7 kb primer was designed,synthesized and used to check the genomic templates for influencing on PCR amplification efficiency by different extraction methods.[Result] The yield and quality of genomic DNA extracted by the silica membrane adsorption method was better than that extracted by the traditional phenol/chloroform extraction method.Moreover,through using the genomic DNA of silica membrane adsorption method as template of Polymerase Chain Reaction（PCR）,the influences of anticoagulants on the amplification efficiency were eliminated.[Conclusion] Silica membrane adsorption method lays the foundation for simple and rapid extraction of high quality genome DNA from avian blood.

关 键 词：家禽血液 硅胶膜法 酚/氯仿抽提法 聚合酶链式反应 

分 类 号：Q78[生物学—分子生物学]